...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...they are directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame...

...alone; a researcher doing some experiments in Brazil can have the same issues as one doing experiments in ...sometimes wish they could live differently than we do day to day. We discovered that it can be good for...

...“Synthetic biology, in particular, the ability to do DNA synthesis, is evolving so fast that it is a really...technological change happens really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic... engineered? Where did it come from? What does it do? Those are the sets of questions one would want to...

...with a PhD in biology. Depending on what you want to do, that’s not necessarily bad news, but it is an argument... science with the community. Some of them you can do today! 10 ways to share your science 1)   If you ...talk – really talk – about the research you are doing in the lab with your mother or father or your best...

...support your career development as your mentor. How do you make the most of this new relationship? Consider...skills in which you have expertise or knowledge. Where do these intersect? Work with resources: Choose books...take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...

...questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should...the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub. The...

...the population that have an edit. I didn’t do this, but to do it you would need a pretty good gel image...Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal lines (Figure ...expansions (monoclonal, 1-5). Wild-type A549 cells do not express CAs9 and are included as a control (WT...couldn’t get a good image. You could probably also do Western blotting against GFP expression, but I didn...designing primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was...

...How do you know if you actually have DNA in your tube without seeing it? There are many ways to do this... time to set up in the lab, you might not have to do the calculation yourself as many fluorometers will...

...are the perfect person to be writing the review! Doing so is a great way to develop your ability to write...here, I will share my process with you. But before I do that, let me say that I was by no means an expert...overthink it. And to quote my adviser, "you just gotta do it." At the end of the day, someone has to write ...article and that someone is going to be you. So, just do it! I will also paraphrase what I have heard many...

...Satellite colonies are small colonies of bacteria which do not contain the plasmid and its associated resistance...effectively selecting plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative...Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy because ...above antibiotics would work for you, they likely do! Give our summary table a look for a quick recap ...