We narrowed to 783 results for: INA

... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...a simple egg is packed with information. I am fascinated with how developmental programs unfold, absorbing...comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms ...stick to vertebrates alone.  And a longstanding inclination to be a teacher was looking for an outlet. I ...Association for Women in Science); others were visiting seminar speakers. My eye was caught by a couple of unusual...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...

...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly used cell...difficult to determine its true tissue origin. Originally, it was assumed HEKs were a common cell type ... necessary, HEKs are human in origin, so they eliminate non-human PTMs which is a concern with other protein...cultured human cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also...., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol...

...technology. In each section below, we cite the original paper but link to plasmids from the Promega Plasmid...similar constructs available at Addgene. Read the original paper for more detail on whether the authors incorporated... attempts to purify or overexpress proteins inappropriate in discerning their true roles. Work conducted...receptor (EGFR), a transmembrane receptor tyrosine kinase essential for cell proliferation signaling. CRISPR...angiogenesis through its principal receptor tyrosine kinase VEGFR2 (also known as KDR), orchestrating blood-vessel...cb3002478 Kamada, Y., Tateishi, H., Nakayamada, U., Hinata, D., Iwasaki, A., Zhu, J., Fukuda, R., & Okiyoneda...10.1111/bph.15426 Promega Corporation (2025). Illuminating Transmembrane Proteins Using HiBiT CRISPR Cell...

...conductor receptor (CFTR) by homologous recombination in cultured intestinal stem cells of patients with cystic...This post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie...CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to the early...Chinese Academy of Sciences found that mice with a dominant mutation in a gene that causes cataracts could... chose to explore its potential in mice with a dominant cataract disorder caused by a single copy of a... "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients...

...interest: The transgene sequence is flanked by long terminal repeat (LTR) sequences, which facilitate integration...line that stably expresses Gag-Pol and/or Env, eliminating the need to deliver these genes in trans by specific...feature may be useful for the transduction of terminally differentiated (non-dividing) cells. Both lentiviruses...produce additional virus. Are γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN... LTR Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3-R-U5 regions found on either side ...U5 and the rest of the provirus. 3’ LTR Terminates transcription started by 5' LTR by the addition...written and updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook...

...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as Cre-lox allow for more...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and ...and construct are coexpressed. Three new recombinases from the Cepko Lab are now available as AAV1. CRE-...constructs 69570 and 69571 together encode the N and C terminal segments respectively of a GFP dependent Cre. ...to form a functional Cre enzyme that induces recombination of lox sites. This system takes advantage of...

...efficiency.  Finding recombinant psychrophilic enzymes for the host The expression of recombinant proteins for...conditions decrease the potential for microbial contamination. Additionally, the high activity of these enzymes...well under suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as ...would allow the stable folding and expression of recombinant psychrophilic proteins. As the figure below shows... as engineering and regulating psychrophilic recombinant proteins.  To infinity, and beyond! Keeping ...brainstorm with each other. The event also featured a seminar on “Tools to increase scientific reproducibility...

...With no blue light, the channelrhodopsin remains inactive and neurons don't fire. With blue light, ions ...minimally-invasive, Frances Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins... To overcome these challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help...information about parental channelrhodopsins to create recombinant channelrhodopsin sequences. The model first removed...their success in vivo. Therefore, the Arnold and Gradinaru Labs expressed ChRgers in mice to see if they ...broad transduction of the nervous system which eliminates the need for intracranial injections. When packaged...Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin...

...second day of Antibody, Addgene gave to me…  A recombinant anti-GFP!    🎶On the third day of Antibody, ...fourth day of Antibody, Addgene gave to me…  A recombinant Anti-Arl13b!    🎶On the fifth day of Antibody...sixth day of Antibody, Addgene gave to me… A recombinant Anti-PSD-95!    🎶On the seventh day of Antibody...eighth day of Antibody, Addgene gave to me…  A recombinant Anti-DYKDDDDK!   🎶On the ninth day of Antibody...tenth day of Antibody, Addgene gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody... of Antibody, Addgene gave to me…  A growing recombinant antibody collection!     ...

...Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying...tRNA suppression competes with natural chain termination at stop codons, expression levels of these tagged...acceptors, then biarsenicals are a good option. Originally developed by Roger Tsien and co-workers (Griffin...compounds such as British anti-Lewisite (which was originally developed to treat heavy metal intoxication in...insert your protein of interest, resulting in a C-terminal fusion of a tetracysteine tag. In addition, since...so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental design...Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside live cells." Science281.5374...