...Ligation Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective...Resources on the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques ...Techniques Additional Resources on Addgene.org Find Plasmid Cloning Protocols Find Virus Protocols Find Basic...

...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened...HyPer3 (2) which he will co-express together with a plasmid encoding glycine oxidase. The goal is to determine...excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to...Bacterial Expression Systems Learn about Global Plasmid Distribution ...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT...’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase (RT...polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157... cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with...fluorophores. Find all of the nanobody toolkit plasmids here. Buser DP, et. al. PNAS 2018. PubMed PMID...

...What SARS-CoV-2 plasmids from Ginkgo Bioworks are available for request? Currently, plasmids containing all...available. Plasmids are continually added as they complete QC and you can find all available plasmids listed...to fresh data! These same plasmids are then added into our normal full plasmid NGS queue in order to bring...pipeline. For the first ~50 plasmids we perform our standard full plasmid sequencing by NGS. Addgene’s...on Addgene.org Find all plasmids from Ginkgo Bioworks  Find COVID-19 plasmids for your research Browse...Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ... to synthesize and share over 1,850 SARS-CoV-2 plasmids to help academic and industrial labs accelerate...

...additional hot plasmids in our newsletter or on our hot plasmids webpage.   Hot plasmids covered in this...Segmennt of Improved CRISPR Base Editors Hot Plasmid 4: pPSU Plasmids for generating DNA molecular weight markers...In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling...this segment Hot Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes with light.... Listen to Our Segement on PhoCl Segment  Hot Plasmid 2: Ouabain selection for increased CRISPR editing...Listen to the Segement on Ouabain Selection  Hot Plasmid 3: CRISPR base editors with enhanced range and ...markers.  Listen to the Segement on the pPSU Plasmids Special thanks to our sound engineer/producer, ...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit ... achieved with classical gene overexpression and knockout techniques. Find the plasmids from Aranda-Díaz...sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted endogenous protein...your protein knockdown experiments! The TRIM-Away plasmids can be found here. Clift et al. Cell. 2017. PubMed...Endosomal pH Sensors podcast segment The first Addgene plasmid deposit from Professor Stefan Hell, who won the...

...and to model diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells...suitability in iPSC programming spanning from fibroblasts and keratinocytes to peripheral blood mononuclear...patients in contrast to the process of collecting fibroblasts, which require a more invasive skin biopsy procedure...larger number of willing participants. While fibroblasts obtained from aged patients are reprogrammed ...and the subsequent reprogramming success [1]. Fibroblasts need to be cultured and expanded in vitro for...signatures that are closer to hiPSCs than age-matched fibroblasts [3]. These advantages make PBMCs the ideal source...Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors To date, the...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        New toolkit... the Kanemaki lab has created a new toolkit of plasmids for the N- or C- terminal tagging of proteins ...interest to create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a...previously difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018....datasets for research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications...

...interest has been cloned into an expression plasmid, the plasmid can be introduced into host cells, such ...that the plasmids and sequences for antibodies are not shared. For instance, through open plasmid sharing...Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing one ...synthetic genes that are typically expressed from a plasmid or from an integrated sequence in a stable cell...heavy chain genes of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an...laboratories and companies have already begun creating plasmids encoding affinity reagents. These include conventional...performed will be useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral...treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International...promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications,...