...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which SNPs ...ancestry-based bias.   The correction process does not stop there. Currently, they are working with Broad's ...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...

...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...to inject an Agrobacterium culture through the stomata into the abaxial site (lower surface) of the leaf...MoClo system you can easily and quickly create customized vectors. For more information on MoClo for plants...is a PhD candidate at Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology...

... payoff for each aim. Learn to communicate your story with only the most salient details. Make the most...your research problem. Creating a coherent unified story and model can help make your ideas easier to understand...Practice presenting your chalk talk Perfect your story and delivery. Design simple figures and diagrams...track. Furthermore, practice getting back to your story. Committee questions can derail your talk. You need...

...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent...

...infectious titer. The infectious titer of a viral vector stock can vary based on transduction target and can also...particles were full, compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-...100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center for Nanoscale Systems, Harvard ...Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization method (DNA...

...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development...scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when ... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

... done at my first six ASM meetings. I told this story over a great dinner at Pittypat’s Porch with old... career scientists, students, and those from historically underrepresented groups. Through such mentoring...Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa ...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...editing treatment would have to be individually customized to the patient's specific mutations. With a drag...Kaiyi Jiang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P....

...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially... Transfer    Interesting Articles About FRET: Piston DW, Kremers GJ. Fluorescent protein FRET: the good...

...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a stable...affecting many of the RFPs developed from natural ancestors), and screened through libraries of new RFP variants... an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon...entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were carefully...