...flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies ...multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary antibody...this blog post has answered all of your questions about the utility of isotype conversions and chimeric ..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...

... with the three other organizers. We asked them about their experiences as they navigate the new norm ...pre-workshop questionnaire to the participants to learn about what they were looking to learn in the workshop ...having a stable internet connection,” says Abitua about her workshop. “We made sure that we had a backup...on the Addgene blog Read all of our blog posts about conferences Find 25 tips for working from home ...

... to find that there is insufficient information about which reagents were used? Unambiguous identification...available through Addgene and find verified information about each plasmid item. Given a specific Addgene ID #...the Addgene website and find specific information about the plasmid: name, description, how the plasmid ...out this post on Scientific Reproducibility Learn about the anatomy of a plasmid page at Addgene Additional...

... in culture and in vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer...within the near-infrared (NIR) spectrum (>620 nm). Above is a brief schematic of how the MG-2I-dL5** FAP-...use MG-2I-dL5** with your research? Let us know about your experiences using photosensitizers in the comments... to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org...

...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

...posts on base editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series...both changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased...change C to T, G to A, A to G, and T to C, represent about two-thirds of known pathogenic point mutations. ...

...Addgene blog Learn about using nanobodies to control protein activity Read about RANbodies, reporter ...reporter nanobody fusions Learn more about using the secondary nanobody toolbox for immunodetection Resources...Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics guide ...

...teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you tell me where to start... for scientific exploration, discovery, and collaboration. One module currently under development comes...circuits and logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder book chapter... or small, receive the BioBuilder textbook, a laboratory kit, and support during the academic year from...

...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns...Nano-lanterns, Takai et al. continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL)... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check Out ...

...the name, date, and location of all analyses (see above). Give every experiment its own directory: In ...record your goal, approach, and conclusions (see above). Keep original files in a separate folder: You... can simply make a note in your methods section about the edit that was temporarily used. Just be sure... Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time and Track Your...

...Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended the 2018 Chromosome...lot of scientists who not only remember to think about DNA in this context, but also specifically study...plasmids containing these genes. As with the PIs above, many of these genes were discussed at the 2018 ...chromatin This is not the first Addgene blog post about chromatin--multiple Addgene colleagues and guest...

...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...this paper are available at Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources... Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional...Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org: Find...

... Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...We often think about the brain’s function in terms of its regions. But equally important is the way the...head bone’s connected to the neck bone,” but with about a hundred billion neurons and six hundred trillion...

...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

... called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed through NABOR are recombinant...troubleshooting methodologies drew me to learn more about the process that was used to create this important...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

...from previously discussed methods. As described above, you design two primers, each containing the desired...suited for insertions and deletions. The figure above shows guidelines for primer design; a desired insertion...mutation-containing colonies/total colonies) is about 84% for each of the three methods. However, REPLACR-mutagenesis... Additional Resources on the Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden...

...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...

...that screen with a system you are really confident about working for most genes and individual targeting ... system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could...RNA we use. What we have to do now is learn more about the rules.” He said that there is no theoretical...References & Further Reading: For more information about SAM, including a tool for getting optimized guide...

...transitioned away from the bench and became all about finding the best product-market fit for this set...Notably, the team must have an upfront conversation about its goals and vision for the company; this will ...section! And read on for more great information about starting a biotech company. Suggested reading The...of Sample6) for taking the time to speak with us about the biotech startup process and work environment...

...fragment are we talking about here? Many of the Fab fragments described above can be used for similar ...fragments are produced by proteolytic digestion above the hinge-region, which results in two independent...you consider which tools to use, be sure to think about what pieces of the antibody you do and don’t need...