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Showing: 421 - 450 of 535 results
  1. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...Additional Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins...
  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...other AAV. Therefore, one should aim to keep their cargo below 4.4kB (with the ITRs bringing total genome...
  3. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature...
  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...
  5. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...proteins will refold once denatured, however, so take care when using this method.      Fig 2:  A, ...
  6. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...proteins of interest - the secondary antibody needs careful consideration before selection. If all the primary...
  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...
  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...distribute through our deposit process, and then we carefully track samples through our lab using barcoded tubes...
  9. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...efficiently enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged...
  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...cells. What’s more, G-baToN can be used to bring cargo, such as protein or DNA, to manipulate function ...
Showing: 421 - 450 of 535 results