We narrowed to 801 results for: DES

...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...delivery of the actual virus to which immunity is desired. Instead, components of the virus are now delivered...

...target cells and organisms, and when that cargo is designed to make you sick…well, you feel it. But the same...host genome or temporarily express whatever it encodes. This ability to infect cells with a high efficiency...

...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ... popular light-regulated two component systems designed by the Tabor lab. Which inducible promoter is ...

... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ...

...control, generates  educational materials, and provides viral vector technical service (details about ...tracking. Our Software and IT Teams made major upgrades to our inventory management systems to enable ...

...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant...Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request...

...and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is...mutations in one of these parts, the GNB1 gene, which codes for G𝛃1 subunits of G protein complexes, lead to...

...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362...

... other sources. Mode of communication Different modes of communication take up different amounts of mental...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

...showing the potential for broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit...tracing Article contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases...

...sulfurtransferase (MPST) which catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound...transferred to thiol-containing acceptors. These hydropersulfides modulate protein activity and have important...

...electrical activity in the brain by inserting electrodes into the brain. But this process is time consuming... the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP...

...ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our shipping ...most popular website in the world. I know, right? Upgrades to the internal LIMS pages and excellent work ...

...of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To keep gene expression stable...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic...

...overcomes this limitation and allows for the flexible design and small scale production of antibodies. Problems...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1...create other photoswitchable kinases? The general design of psMEK1 also worked for creating three other ...

...Amsterdam. The previous two posts in this series described a practical approach to selecting a bright fluorescent...preprint, or documenting it in some other way. Besides the data, new plasmids and/or cells or organisms...

...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

...behavioral neuroscience, and address experimental design considerations used in all behavioral studies. ...ion channels. Similarly, chemogenetic tools are designed channels that are chemically activated. Both are...

...reporter gene, which replaces the targeted gene and provides a way to track successfully engineered cells. ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...

...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test...

...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... because sometimes companies have relaxed dress codes for interviews. You can also ask HR for clarification...

... but educators face significant challenges in designing suitable projects. In the O'Connor lab, we recognized...Over Time project The Pathways over Time project provides a framework for these undergraduate research questions...

... Guardian, BBC Breakfast or the Londonist. BBC provides a peak into their day-to-day business with popular...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions...

...virus will not only ensure that your infection is designed in the best possible way, but it may also save...the transgene itself. Many researchers prefer to design primers that target the common features of viral...

...plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized by Dmitry...CRISPR regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against ...

... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...

...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery...stepping into a sea of posters – where do you begin? Design a game plan Prior to attending the conference, ...

...plasmid recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can...exchange will prevent your cultures from reaching the desired density. The volume of your flask or culture tube...

...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...and acquire images of a sample from two opposing sides and merge the images, such as in the Ultramicroscope...

...resistant to disease. They also devise biosensors designed to elucidate the molecular interactions that go...things for day-to-day life in a plant lab, so it includes everyone’s favorite promoters, plus a few new ...

...case, a buffer exchange can be performed using a desalting column or an ultrafiltration concentrator. As ...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

...improve antibody binding for some targets, they can destroy it for others. That said, organic solvents’ impact...critical to the outcome of your IF experiment and deserve careful thought and attention.   * There are other...

...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that... Image: Kim and Morrison, 2009. This method provides DNA yield and quality similar to that obtained...

...LLC, a grant professional development firm that includes the social media community GrantChat. She has ... from administration and fundraising to program design and evaluation. She and her team at J. Miller &...