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  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362...

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant...Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request...

  5. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...overcomes this limitation and allows for the flexible design and small scale production of antibodies. Problems...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

  6. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Solution: In Zhou et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1...create other photoswitchable kinases? The general design of psMEK1 also worked for creating three other ...

  7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...Amsterdam. The previous two posts in this series described a practical approach to selecting a bright fluorescent...preprint, or documenting it in some other way. Besides the data, new plasmids and/or cells or organisms...

  8. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... other sources. Mode of communication Different modes of communication take up different amounts of mental...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...using a resistance marker; however, it is often desirable to remove the marker after the initial selection... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... because sometimes companies have relaxed dress codes for interviews. You can also ask HR for clarification...

  12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...reporter gene, which replaces the targeted gene and provides a way to track successfully engineered cells. ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...

  14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... Guardian, BBC Breakfast or the Londonist. BBC provides a peak into their day-to-day business with popular...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test...

  16. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...the transgene itself. Many researchers prefer to design primers that target the common features of viral...

  17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized by Dmitry...CRISPR regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against ...

  18. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...

  19. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery...stepping into a sea of posters – where do you begin? Design a game plan Prior to attending the conference, ...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...plasmid recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can...exchange will prevent your cultures from reaching the desired density. The volume of your flask or culture tube...

  21. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...and acquire images of a sample from two opposing sides and merge the images, such as in the Ultramicroscope...

  22. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...resistant to disease. They also devise biosensors designed to elucidate the molecular interactions that go...things for day-to-day life in a plant lab, so it includes everyone’s favorite promoters, plus a few new ...

  23. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that... Image: Kim and Morrison, 2009. This method provides DNA yield and quality similar to that obtained...

  24. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  25. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

  26. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...improve antibody binding for some targets, they can destroy it for others. That said, organic solvents’ impact...critical to the outcome of your IF experiment and deserve careful thought and attention.   * There are other...

  27. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...case, a buffer exchange can be performed using a desalting column or an ultrafiltration concentrator. As ...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  28. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...LLC, a grant professional development firm that includes the social media community GrantChat. She has ... from administration and fundraising to program design and evaluation. She and her team at J. Miller &...

  30. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is... may be unintentionally mutated along with the desired target locus. The PAM sequence is of particular...

  31. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic acids with high sensitivity...per second. A quantifiable fluorescent signal designates the presence of your DNA of interest, in this...

  32. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead...Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility caused...

  36. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...staining. Antigen-containing samples often include undesirable molecules that may bind non-specifically to assay...specific than other methods. In addition, the assay includes both primary and secondary incubation steps, increasing...

  37. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

  38. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been...symbol of opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas...

  39. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab has shown some...

  40. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...
Showing: 561 - 600 of 786 results