...number during the transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein...plasmids modified from pSC101 with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered...

...example, IZKF1 and IZKF3 are cell-type specific transcription factors with important roles in Multiple Myeloma... (Nabet et al., Erb et al., Huang et al.), transcriptional and epigenomic profiling studies (Nabet et ...Featured molecules: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia...

...the embryos underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma...maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

...not by Cranfill et al (2016)). Moreover, good performance in the OSER assay is not a guarantee that using...fluorescent proteins when protein tagging is the goal (Cranfill et al, 2016; Constantini et al, 2012). The localization...

...poison, locking up DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene... that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used...

... 3’ end. This repeat region associates with the trans-activating crRNA (tracrRNA) which interacts with... important cellular RNA molecules with post translational modifications. One of the most common of these...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings...

... Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR,...695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki...

...position, I just had a phone interview. I also transitioned between different lab groups without having ... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...experience less stressful. First, you can make arrangements with those you live with to have a quiet place...

...that the TadA domain can edit the short single-stranded DNA stretch that is accessible. Image from Gaudelli... protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher...References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base ...

...loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout that can... 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs from...toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step...

...Interactions Difficult to detect weak and/or transient PPIs. Many PPI identification techniques rely ...often prevents the identification of weak and/or transient interacting proteins. No spatiotemporal control...BioID, is also useful for identifying weak and/or transient protein-protein interactions since the biotin ...

...pipetting error or an incredible discovery. The range of RQ values used to call copy numbers should be...fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an intercalating dye, with...confident determination of copy number, albeit within a range of RQ values as noted above.   In both applications...

... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...

...of the endonuclease Cas9 to create a DNA double stranded break (DSB) at a genomic site specified by a guide...tag. The two PCR reactions are then mixed and transfected along with a Cas9 expression vector. They can...interactions. Additionally, thanks to the modular arrangement of vectors included in tagging systems like SapTrap...

...to choose  Where is your audience?  It’s, quite frankly, much easier to go where your audience is than ...resources do you have?  No matter the project, I can guarantee you that either resource or time restrictions ...competitive while digital can be oversaturated. Transition from academic to informal writing can difficult...

...jam packed with everything needed for synaptic transmission, are right around 250 nm, making it difficult...pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should...brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110. https://doi.org/10.1098...

...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate... now connects MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...

...enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful experiments...“GPS for proteins”) for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality...

...control. A good positive control is bacteria transformed with the same backbone plasmid. If this control...strain. A good negative control strain is an untransformed culture of the same strain of bacteria you used...Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ...

...diligent scientist, they sought to understand these strange results by digging deeply into their data.   ...Samples are divided into those of African ancestry(orange) and those of other ancestry groups (grey). ...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...

...responsibilities. Another tip: don’t miss your grandfather’s 100th birthday, as your mom will never forgive...when you can’t get home, babysitting or running errands. Give help when you have time, and in return you...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

...result from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be ...file. On the plot, the green (edited sample) and orange (control sample) lines should be close together...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...

...paid on fellowships instead of grants such that PIs no longer save grant money by paying relatively low...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan...

...using the actual tool that you ordered and not a random aliquot pulled from a freezer. As you’ll see in...review our experimental designs and we need to be transparent about the reasons we alter these designs if we... wherein researchers can submit designs and transparently reveal them upon completing an experiment. There...

... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...

...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...

...Subscribe to the blog to stay in the know!     No pranks, just fun and games this April Fool’s Day on the... to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each photo, the pet...

...competitive field. Chapter 2: Wei Leong Chew's swift transition from graduate work to an independent position... Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: Wei Leong Chew's lab...

...Troubleshooting Viral Transductions Getting the Most from Your Lentiviral Transduction Additional Resources...

...100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown announced...substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will allow us to...

...the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History Rap Battle...telling following the unfortunate story of Rosalind Franklin and her incredibly important role in solving the...

...field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook....other Cas proteins continue to expand the targeting range. CRISPR can be difficult to deliver. Not all cells...

...Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf... care package from Addgene. We hope all of our entrants had fun decorating with their labmates! ...

...diverse needs of our community and support a wide range of experimental designs. Here are nine great additions...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

...Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4 - Developing...ensuring that success Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...

...scientific breakthroughs, reagents, and know-how to transcend national borders and help people around the world...accelerate biology research in the labs they’re granted to, as well as shine a light on some of the great...

...light chains and two heavy chains. The ends of the branches contain the antigen binding sites and are highly...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...