We narrowed to 850 results for: abo

...in the next few months! Maybe our favorite thing about our new logo is what happens when you repeat it....Blugene’s new look symbolizes the innovation and collaborative spirit at the heart of Addgene. Embodying the...

...us with AAV testing and recently had this to say about the service: "I'm excited that Addgene is now...other expression data. If you have any questions about the Viral Service or any of the materials we distribute...

...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

...getting together and chatting with another person about their career path is a great start to a mentoring...Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor: The...

...populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions...of days. Addgene: Can you tell us a little more about this new two-color tool and what it can enable? ...

... who aren’t familiar with it. If you’re curious about the hows and whys of the material transfer agreement...easier for others.  If you’ve still got questions about our MTA process, you can check out our Tech Transfer...

...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...

...brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...sensor). In preliminary studies with the lab’s collaborators, they were able to use the serotonin sensor ...Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light and temperature...

... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

...skills and experience.You might not normally think about it, but hobbies and volunteer experiences, as well...show that you have some of the abilities listed above. Showing that you are willing to exercise these ...

...conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions...

...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager...

...clarifying roles, I eased the tension and improved collaboration. The good news: It’s a journey, not a final...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

...interest. Created with biorender.com. The plot above is for a smeary expression and is quite different.... Additionally, you can gate off the histograms above, allowing you to visualize your data as a dot plot...

...packaging methods. The three components described above (envelope, packaging, and transfer) are supplied...gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...

...component of a biosensor is the thing that you care about within your cellular or animal system. This ‘thing...for expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We ...

...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... that are otherwise cut off from the process.   About the author: Benedict Diederich received his PhD ...

...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...blog to learn about making your own competent cells and our protocols page to learn about bacterial transformation...

...labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get phenotypes...coverage. Please see this paper for more information about PHP.eB brain labeling at various doses.  As always...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used...

...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome Engineering...

... single amino acid. Without further information about properties like photostability or aggregation tendency...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources...

...targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing.  For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below...

...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

...with limited packaging capacity. Still confused about the various tRNA expression strategies? No worries... Addgene. If you have any questions or thoughts about this technology, leave them in the comments below...

... extremely heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation...favor accumulation of the latter products. As noted above, a single cycle of cleavage and accurate repair ...

...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...writing! Let us know if you have any other questions about writing and publishing your manuscript, or add your...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...were observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9...

...Fluorescent 101 blog posts Read about chemogenetics vs. optogenetics Find more about the many types of protein...

...power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

...talking about Click Chemistry since 2001, making use of robust reactions that work in just about any situation...