We narrowed to 812 results for: APP

1. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   Published

   May 12, 2015, 1:32 p.m.

   ...tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase...

2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

   Type

   Blog Post

   Published

   July 29, 2014, 6:16 p.m.

   ...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...

3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ... lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can...

4. Antibodies 101: Fc Effector Functions

   Type

   Blog Post

   Published

   May 18, 2023, 1:15 p.m.

   ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in...

5. CRISPR 101: Drug discovery and CRISPR

   Type

   Blog Post

   Published

   May 4, 2023, 1 p.m.

   ...discovery Present drug discovery takes one of two approaches. The first route involves identifying a gene/...

6. Cellosaurus: A Cell Line Information Database

   Type

   Blog Post

   Published

   Feb. 21, 2023, 2:15 p.m.

   ...cells lines. A 2014 study in Science found that approximately one-third of cell lines used in scientific research...

7. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative...

8. Plasmids 101: What is a plasmid?

   Type

   Blog Post

   Published

   April 2, 2020, 6:17 p.m.

   ...broadly utilize plasmids across a wide range of applications. Some common plasmid types include cloning plasmids...

9. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

   Type

   Blog Post

   Published

   Jan. 21, 2020, 2:30 p.m.

   ...publication are also provided for the user. This approach serves to maintain consistency with published ...

10. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    Published

    March 15, 2018, 12:59 p.m.

    ... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...

11. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type

    Blog Post

    Published

    Nov. 1, 2017, 2:04 p.m.

    ...making it accessible to students.” Making science approachable is often the most challenging part of the job...

12. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type

    Blog Post

    Published

    Oct. 18, 2017, 1 p.m.

    ...upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job search sites...

13. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    Published

    Nov. 29, 2016, 3:30 p.m.

    ...motion format for our videos as it is the most approachable for LEGOs. Stop motion consists of taking many...

14. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type

    Blog Post

    Published

    June 5, 2017, 3:19 p.m.

    ... and maintaining confidentiality where it is appropriate. This grows our relationship with our community...

15. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type

    Blog Post

    Published

    June 19, 2018, 1:38 p.m.

    ... experiment to demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1...

16. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type

    Blog Post

    Published

    Aug. 22, 2019, 12:43 p.m.

    ...resistance genes, toxin genes, and the sacB gene). By tapping into nature’s toolbox, scientists are finding more...

17. Sensing Neuronal Dopamine

    Type

    Blog Post

    Published

    July 30, 2019, 12:59 p.m.

    ...neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it’s also involved in reinforcement learning...

18. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type

    Blog Post

    Published

    July 16, 2019, 12:59 p.m.

    ...spontaneous swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new...

19. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type

    Blog Post

    Published

    June 11, 2019, 1:16 p.m.

    ...Commons 3.0 license, which requires users to give appropriate credit, provide a link to the license, and indicate...

20. Networking and Inspiring Young Scientists at Freshers Fairs

    Type

    Blog Post

    Published

    Nov. 17, 2015, 3:30 p.m.

    ...sessions to help undergraduate students with their applications. The society currently has 1,283 members, who...

21. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type

    Blog Post

    Published

    July 2, 2015, 8:37 p.m.

    ...follow your passion and land a position you’ll be happy in once you’ve finally acquired that PhD. Resources...

22. 10 Ways to Share Your Science!

    Type

    Blog Post

    Published

    May 13, 2014, 8:34 p.m.

    ...Morehead Planetarium. Similar things are likely happening in your community, and, if they aren’t, think ...

23. Choosing a Good Mentor for Scientists

    Type

    Blog Post

    Published

    Jan. 16, 2014, 2:56 p.m.

    ...away. Many scientists think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad ...

24. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ...interfaces with the entire body, so the obvious next applications are to expand the tool to different brain-organ...

25. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ...published, knockout validated, and work in your application. But…they are the same isotype and therefore ...

26. RUBY-Red Siliques

    Type

    Blog Post

    Published

    March 1, 2022, 2:15 p.m.

    ...culture), which is great for a wide variety of applications. But some techniques require you to select against...

27. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...several MYC-dependent cancer cell lines. It can be applied for identification of essential MYC binding sites...

28. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...reached the level of expression needed for most applications.   Figure 1: With blue light, the VVD...

29. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    Published

    May 25, 2021, 2:29 p.m.

    ...which can limit expansion into new services. “We appreciate NIH’s vision and support for this project. This...

30. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    Published

    June 2, 2020, 1:15 p.m.

    ...population, it’s easier to visualize individual/non-overlapping cells. Until now, tools for doing this were labor-intensive...

31. Using Addgene's New Viral Service for Your Research

    Type

    Blog Post

    Published

    Nov. 17, 2016, 3:30 p.m.

    ...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...

32. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type

    Blog Post

    Published

    Jan. 24, 2019, 2:24 p.m.

    ...PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal circuitry with an index-optimized clearing...

33. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    Published

    Jan. 22, 2019, 2:41 p.m.

    ...on counting the number of autophagosomes. This approach, however, has inherent limitations because it ...

34. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    Published

    Sept. 18, 2018, 1:33 p.m.

    ...cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature...

35. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    Published

    Aug. 8, 2019, 1:19 p.m.

    ... understand or individual words that are not appropriate for the given context. After this first read,...

36. Summer SciComm Series: Master of SciComm

    Type

    Blog Post

    Published

    July 21, 2022, 1:15 p.m.

    ...science communicator—with an understanding of how to appeal to a given audience and how to utilize a wide array...

37. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...

38. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

39. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...you choose could be based on your downstream application, time, and instrument availability. UV absorbance...

40. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    Published

    Dec. 10, 2019, 1:58 p.m.

    ...The LipoGlo system represents a new, intriguing approach to expand our understanding of ApoB-LPs and accelerate...

41. Cultivating Community Science at BosLab

    Type

    Blog Post

    Published

    Nov. 2, 2017, 1:35 p.m.

    ...the biotech revolution. Our lab exists in what appears to be a funky old garage, but looks can be deceiving...

42. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ... we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for...

43. With Our New Viral Service, We're Taking CRISPR Further!

    Type

    Blog Post

    Published

    Dec. 6, 2016, 3:30 p.m.

    ...Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids for ...

44. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition...

45. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...by TUNEL staining.  The video below shows what happens to the zebrafish’s heartbeat and blood circulation...

46. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...

47. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ... an upright position for 30 seconds, and then recapping the tubes prior to thaw. We compared vented and...

48. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    Published

    Aug. 2, 2018, 12:56 p.m.

    ...mix chemicals together in chemistry to see what happens, but there isn’t really an equivalent of a “chemistry...

49. New and Upcoming Viral Vectors - June 2019

    Type

    Blog Post

    Published

    June 18, 2019, 1:15 p.m.

    ...ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory of DREADDs / Chemogenetics...

50. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    Published

    Aug. 2, 2022, 1:15 p.m.

    ... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...