We narrowed to 801 results for: DES

...harder to build relationships when you are more desperate or needy so you can’t wait until you imminently...

... enhances productive collaborations as well as provides visibility for grant-driven research being done...

...this post, you’ve got the knowledge to be able to design a construct for the successful and specific expression...

...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

...immunocytochemistry (ICC). Not all of the solutions described here may be as effective for other techniques,...

...In the case above, you can’t costain with your desired antibodies because the pair belong to the same ...

...someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal forms of ...

...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...

...activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to...

...dynamic process into a static measurement and it provides limited information about what materials or organelles...

...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

...needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction...

... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...

...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...

...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...

...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

...on Twitter where each poster presenter had four “slides” to use for their poster, eliminating the need ...

...retroviral CRISPR knockout library using gRNAs designed by the Kosuke Yusa Lab. This retroviral library...

...bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic binding...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...

...on social media or the phones hiding under their desks than listening to me. When I got home, I saw my ...

...next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300 bp sequencing products...

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to...

...of R bodies R bodies have been studied for many decades, and they were known to be resistant to detergents...

...Define the scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab ...

...University of British Columbia, Student Services - includes videos on general resume writing, as well as additional...

...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate to ...

... that you know how to read flow plots and have designed your first flow panel, you’ll load your samples...

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

... from University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs)...

...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

...about a specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV....

...Bhopal. All illustrations / figures featured were designed by our team member Kallol Mozumdar. Sreepadmanabh...

...even if science writer Ed Yong’s book “I Contain Multitudes” has me considering the microbiome more often...

...different phenotypes. For example, this plasmid encodes a green fluorescent protein gene which makes the...

...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

... don’t overdo it (some of my best studies were designed over late night poker games at Gordon Conferences...

...have been a model organism for plant research for decades, and the biochemical pathways involved in central...

...talking about here? Many of the Fab fragments described above can be used for similar applications and...

...affinity, many indirect antibody protocols are designed to take place over multiple days. Increased run...