We narrowed to 868 results for: REN

...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22...

...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...

...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...

...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...

...things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule so you can rely ...Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential publications...

...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...

...the game to each a variety of subjects at many different education levels. If you modify BUILD YOUR PLASMID...us at: [email protected] Marco Straccia is currently an Associate Professor at University of Barcelona...

...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...

...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...

...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...

...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input...

...the interview process was several short talks by current faculty members and it just blew me away….  I have...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...

...interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...

...projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

...optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey!  References and Resources More resources on Addgene’s blog...

...illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ...

...peptide. By combining three different localization peptides with four different fluorescent proteins, and...

...and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ...

...contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be...

...your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania...

...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

...and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering...

...transfer, each GOI is usually expressed from a different plasmid, which may lead to unequal gene dosage...consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all possible combinations...

...organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the...

...probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant...

...fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what...

... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based...

...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ...

...more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang...

...2020). The generated ABE8s needed to produce 3 concurrent base edits to withstand antibiotic selection....great addition to the base editing toolbox.  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran...

...-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance...

...experiment. Good luck with your experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang...dCas9-CBP and the SAM system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-...

... discrimination is especially important in the current outbreak because of the neurological symptoms associated...applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection...

...immunoprecipitate the entire complex in three different cell lines. The Gräslund lab plans to continue...of indels to measure time. These plasmids with different promoters driving expression of Cas9 were used...

...science. It's open-source. You can see all the current code on GitHub, ask us questions about it, or send.... It builds on existing popular web services, currently Crispor and Crispresso, that adhere to the same...

...will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids...light on previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “...

... new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our Plasmids...solutions necessary for advancing the field. References: 1. Internal initiation of translation of eukaryotic...

... acidic, or synaptic vesicles). Some FPs have different ex/em spectra (e.g. mKeima) or change fluorescent...Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: Stepanenko et. al. (2008) “Fluorescent...

...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic...

...decorative" title="Check icon" %}   References and Resources References Hwang, J. M., Piccinini, T. E., Lammel...

...about science whenever he's not off the grid.   References Bischof J, Duffraisse M, Furger E, Ajuria L, ...plasmids and resources Visit our Molecular Biology Reference ...

...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....

...protocol you don’t understand the purpose of, or a different way of doing something then you’ve done previously...an active partner Everyone’s learning style is different, so let your mentor know what yours is! For instance...

...address a diverse set of biological inquiries. References Ogura, Teru, and Sota Hiraga. "Mini-F plasmid... Addgene.org Check out our molecular biology reference Search for a plasmid here Find plasmids from our...

...editing efficiencies in a number of cell types at different genomic loci — in some cases, over 20-fold higher...prime editor. B, Prime editing efficiencies at different loci in U2OS cells. Image adapted from Yan et ...

... by Ina Ersing in August 2023. Resources and references Resources at the Addgene blog Find and share ...Quantification Viral Vectors 101: An Introduction to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson...