We narrowed to 745 results for: TRI

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

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   June 12, 2014, 3:30 p.m.

   ...which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

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   Dec. 7, 2020, 2:53 p.m.

   ...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...

3. CRISPR 101: RNA Editing with Cas13

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   Blog Post

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   July 31, 2020, 12:30 p.m.

   ...these systems in your research? Cary Valley contributed to updating this article. References Cox, David...

4. A Guide to Starting Your Own Journal Club

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   Blog Post

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   Feb. 6, 2020, 3 p.m.

   ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

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   Oct. 10, 2023, 1 p.m.

   ...immediately after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period...

6. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

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   Feb. 9, 2023, 2:15 p.m.

   ... students work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school...

7. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

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   Feb. 27, 2018, 2:04 p.m.

   ...with the desired fluorophores. In figure 4B below, triple colocalization is possible even when staining cells...

8. Tips for Screening with Yeast Two Hybrid Systems

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   Blog Post

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   Oct. 22, 2015, 2:30 p.m.

   .... Lowered expression levels will increase the stringency of your binding. And most importantly: Independently...

9. Plasmids 101: Protein tags

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   Blog Post

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   Dec. 11, 2014, 4:26 p.m.

   ...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...

10. CRISPR Antimicrobials

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    Blog Post

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    May 3, 2016, 2:30 p.m.

    ...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving CRISPR...

11. Droplet Digital PCR for AAV Quantitation

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    Blog Post

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    Jan. 16, 2020, 2:18 p.m.

    ...viral genome (Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must ...

12. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post

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    July 29, 2025, 1:15 p.m.

    ...However, a key limitation of viral vectors is their restricted cargo capacity, which makes it difficult to accommodate...

13. Hot Plasmids: Fall 2024

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    Blog Post

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    Nov. 21, 2024, 1:30 p.m.

    ... & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency...

14. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

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    Feb. 17, 2022, 2 p.m.

    ...that this system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It...

15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

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    April 2, 2019, 1:44 p.m.

    ...and Frasch, 1993).  Tinman, which is spatially restricted to the dorsal mesoderm, is necessary to activate...

16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

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    July 7, 2020, 1:15 p.m.

    .... APOBEC-Cas9n-UGI targets its preferred trinucleotide motifs (5’-TCA-to-TUA) found within the 43 base-pair...

17. Antibodies 101: Reading a Flow Plot

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    Blog Post

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    Feb. 6, 2024, 3:13 p.m.

    ...of flow cytometry is to use increasingly more stringent markers to identify your specific cell population...

18. Plasmids 101: Codon usage bias

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    Blog Post

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    Sept. 27, 2018, 1:09 p.m.

    ... cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures...

19. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

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    Sept. 13, 2018, 1:55 p.m.

    ...and its subsequent repair. Additionally, the distribution of enrichment and depletion scores for safe-...

20. Plasmids 101: Control Plasmids

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    Blog Post

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    April 9, 2015, 3:29 p.m.

    ...selection of proper experimental controls is not a trivial endeavor, as biological systems have many variables...

21. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

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    April 11, 2017, 2:30 p.m.

    ...vivo assays in which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far...

22. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

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    Oct. 25, 2016, 2:30 p.m.

    ...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold...

23. Tips for CRISPR Gene Editing in Mice

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    Blog Post

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    June 28, 2016, 10:59 a.m.

    ... This post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has...

24. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

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    April 3, 2014, 8:05 p.m.

    ...researchers at the NIH here.  Note: A. Max Juchheim contributed to the writing of this article. Additional Resources...

25. The Advances Behind The World's First Personalized CRISPR Treatment

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    Blog Post

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    Sept. 16, 2025, 1:15 p.m.

    ...editing that made this treatment possible — and may contribute to more in the future. Figure 1: A timeline...

26. Antibodies 101: Beyond Surface Labeling

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    Blog Post

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    June 27, 2024, 1:15 p.m.

    ...When it comes to labeling cells for flow cytometric analysis, the most common method is a cell surface...

27. Transferable Skills Guide: Conflict Resolution

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    Blog Post

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    Jan. 4, 2022, 2:15 p.m.

    ...needed to see and just didn’t have anything to contribute. Also keep an eye out for the phrase “I assumed...

28. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

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    Aug. 15, 2019, 12:30 p.m.

    ...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...

29. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post

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    Sept. 28, 2023, 1:15 p.m.

    ...protein of interest, maybe you want to inhibit it. Restricting the effect to a specific time or location can...

30. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

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    March 27, 2018, 1:32 p.m.

    ...Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs...

31. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

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    Sept. 5, 2017, 1:58 p.m.

    ...This post was contributed by guest bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University...

32. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

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    Oct. 11, 2016, 2:30 p.m.

    ...et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) or sonchus yellow...

33. 10 Steps to a Perfect Science Talk

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    Blog Post

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    Aug. 23, 2016, 2:30 p.m.

    ... right now to capture interesting key results, striking images, creative ideas, and engaging stories as...

34. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

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    May 10, 2016, 2:30 p.m.

    ...cardiac disease. This increased disease risk is attributed to latent HIV reservoirs, chronic inflammation...

35. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

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    Jan. 26, 2016, 3:30 p.m.

    ...skipping is a good option for DMD treatment. Clinical trials have used oligonucleotide exon skipping (OEN) to...

36. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

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    Nov. 25, 2015, 4 p.m.

    ...position 5 years after receiving their PhDs, still strive to become academic researchers themselves(44% of...

37. Transferable Skills Guide: Time Management

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    Blog Post

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    Dec. 5, 2017, 1:52 p.m.

    ...mind for the endpoints of a task or project (with metrics if possible) and, at the outset of a task, I outline...

38. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

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    June 24, 2020, 5:45 p.m.

    ...for Epigenetic Modification   Leah Schwiesow contributed to updating this article. References Haldeman...

39. A Control for All Seasons

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    Blog Post

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    Oct. 5, 2023, 1:15 p.m.

    ...species-on-species staining can be partially addressed with stringent staining protocols and the use of isotype-specific...

40. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post

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    Oct. 2, 2018, 12:56 p.m.

    ...decision. If you don’t see a culture you want to contribute to and learn from, keep looking. The right lab...

41. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post

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    April 5, 2018, 12:42 p.m.

    ...typical efficiencies of 30-60%. Quadros et al. attribute much of this variation to differences in guide...

42. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

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    July 12, 2016, 2:30 p.m.

    ...Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning Qin and Haoyi Wang...

43. Antibodies 101: Validation

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    Blog Post

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    March 24, 2022, 1:15 p.m.

    .../19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view from the ...

44. Plasmids 101: Using Transposons in the Lab

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    Blog Post

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    July 6, 2021, 1:28 p.m.

    ... disease. While transposon insertions can be detrimental, it also drives genomic evolution and is used...

45. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

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    Nov. 6, 2018, 1:12 p.m.

    ...This post was contributed by Kusumika (Kushi) Mukherjee. The ultimate goal in the field of regenerative...