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  2. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...efficiency in both types of bacteria. They also replaced the promoter regulating the gene of interest with...the M24 promoter, allowing it to be removed and replaced if needed. In total, three MCS regions permit ...

  3. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...and to get the important information in the right place on the page. 5) Apply for the job as requested in...Resources representative). Pro tip: if there is no place to upload a cover letter, make your cover letter...

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...scientists to deposit and request plasmids (via web interface, processes, distribution, and customer service...Questions? Feel free to reach out to us on Twitter, Facebook, LinkedIn, email, or even the comments section...

  7. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...of plastic for us now). With the personality in place, everything now is about the plot. This part is ...after all, who working in a laboratory has never faced an odd situation with experiments? Our art simply...

  8. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...tools is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine... REPAIR (RNA Editing for Programmable A to I Replacement) system. Like RNA targeting systems, RNA editing...

  9. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

  10. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...In the second-generation SPARC2 system, HDVR is placed upstream of the second attP site to cleave and ...expression of using the SPARC system. The effector is placed downstream of the rest of the SPARC components ...

  11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...self-promoting—No one is going to tell you to pick up the pace Constant worry about grants and renewals What is... can be an issue in getting promoted (especially race and gender) Interpersonal issues can create interpersonal...

  12. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Incubate 5 Minutes at Room Temperature: You can place your reaction on ice if you are planning to transform...polymerases with proofreading functionality are used in place of Taq to reduce error rates; however, proofreading...

  15. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or...

  18. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...level of intimacy that creates the magic of a safe space. No matter why, now is a great time to find a few...Funding Opportunities Open Education Database: 100 Places to Find Funding For Your Research University of...

  20. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Therapeutics, which was acquired by Ultragenyx Pharmaceutical in 2017. As one of the original employees at...impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the...
Showing: 281 - 300 of 602 results