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... had spent most of my junior year stressed out applying for summer programs. I spent one day at Addgene...internship opportunity taking place next summer.  I applied to Addgene and began the interview process with...that my decision deadline would be extended. I appreciated how flexible Amy was, which was the impression... before stepping foot into the lab itself. I appreciated that Addgene was treating all the high school...

...defining a couple of key terms is a better approach. Also, appealing images and colors draw people in. Drawing...should be. He debated taking a position as an application support/sales representative in industry or a...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also...

...bacteria. Learn more about the different CRISPR applications in plants.     Transgenic yeast as source of...Vietnam Linh Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research... water repositories in Mexico. At Addgene we're happy to be able to support diverse groups of scientists...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

...postings looking for PhD applicants since many will also accept applications from master’s candidates ...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...Principal Scientist or Scientist I/II. Promotions don’t happen overnight though, and it might take longer to advance...

...a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein...in plasmid form. Fig. 1: Summary of the approach and outcomes of the screen for improved genetically-encoded...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more...

...types of yeast expression vectors. Consider your applications and experimental design. You can create a new...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...a variety of other types of vectors for most applications used to study gene function. From making transgenic...

...project that combines an exciting new method or approach with another in a way not done before in your ... - maybe something used in astrophysics can be applied to fungal biology.” Connie Cepko advises students... technology to identify a problem, asking what approach will I take and what can I learn, learning the...importance of choosing a solid research topic and approach. At Addgene, we've heard of labs where new hires...

...proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into the CRISPR...various species and describe an assortment of applications. Despite the ubiquity of CRISPR/Cas toolkit,... multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out...for your research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know...

...opportunity to study it and see what happens when technological change happens really fast,” Thompson says. To...of sequence data. A group of scientists from MIT tapped into this data to learn about trends in synthetic...have generated robust tools that have practical applications in monitoring the accidental or intentional ...

...makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of...Institute focuses on proteomics: developing and applying new technologies to quantify proteins in tissues...Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with specific...used to label all proteins within a radius of approximately 20 nm of a specific DNA sequence in only 1 min...

... typical genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of...since the majority of the viral genome has been swapped for alternative genetic cargo, they can’t reproduce...viral preps available for a variety of broadly applicable and popular viral vectors. Check out our viral...should you opt to make a virus instead of just zapping your cells? That really depends on two things. ...

... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in... same species as your primary/sample, and then applying this mixture to your tissue (Tuson, et al., 1990...After washing away unbound fragments, you can then apply your primary and secondary antibodies as normal....Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

... unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic...Quake Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the following year...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...stability under harsh conditions. One example application from the publication is the removal of unwanted...

... transfer resources (summaries, electronic MTA approval, archive of old MTAs, and more) Does your TTO ...periodically for possible new awardees, and we’re happy to share stats and strategies with tech transfer...executed between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene...executed between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA...

...teamwork and initiative it took to make all this happen with 80% of the team working remotely. We’re extremely...capacity to serve the scientific community. First, we applied for the government sponsored Paycheck Protection...a miraculous grant from Fast Grants, which was approved in a matter of days because they grasped the crucial...on track to succeed at the same time. I began approaching science funding organizations to request support...

...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

...all things almost all of us are programmed to appreciate and understand. We build narratives around the...readers or listeners thus expanding the impact and appreciation for their research. Long story short, telling... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...clear what was difficult and what was easy when appropriate. It might not be scientific to bring emotional...

...revolution in biological research. We’re incredibly happy to see that one of our career posts has made it ..., we hope we can help all researchers achieve happiness in their careers regardless of their end goals...addgene.org. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015 1. CRISPR 101...some common controls and why you might need them. Happy Reading!   Resources on the Addgene Blog Download...

...parasites, to symbionts, to genes and the labels applied to them were vague or contradictory, owing in part...elements. At the time, the use of episome seemed appropriate, especially since the Fertility, or F-factor ...seen as an ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded...Vectors" chapters, and more pictures and pro tips.  Happy reading! Learn more about the history of plasmids...

...Creating a modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR...permission from the Genetics Society of America. Applications of this system Dickinson et al. have developed... in cell culture. In addition to CRISPR/Cas9 applications, SEC selection could replace other, more restrictive...Additional Resources on the Addgene Blog An “elegans” Approach to Better Cas9 Editing Efficiency CRISPR 101: ...