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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 534 results for: CAR

Showing: 281 - 300 of 534 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...larger. And since the library contains many plasmids, care must be taken to ensure that all of these plasmids...powerful research tool, but their proper use requires careful amplification and maintenance of good representation...

  2. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check out this video....Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...

  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...intensity and/or duration. Both of these strategies carry the risk of off-target effects. Harald Janovjak ...of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles...lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to ...

  4. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical copy on hand as... to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing a Lab...

  5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ... be assembled in a unique order with no cloning scars. The Elledge Lab showed that a five-piece assembly...researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...

  6. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...regions of the brain. However, the Cre-dependent AAV cargo would only become activated in the presence of Cre...design and interpretation of results. Take extra care when evaluating how this serotype works in your ...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

  11. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ... disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in ...is abnormally deregulated in TS and can lead to cardiac developmental defects. Eight genes from the X chromosome...

  12. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

  14. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...

  15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...not yet fully understood, so it is important to carefully select the target locations of knock-in studies...systems offer a number of different options. By carefully considering your ncRNA of interest, you should...

  16. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...for follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team...Sciences at Arizona State University. His group carries out research in synthetic biology, nanomaterials...

  17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...the molecular biology to learning how to grow and care for the worms. As an example of one of the problems...cheap using equipment that would otherwise be discarded. For instance, the centrifuge in the picture had...

  19. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of ligations in an hour or less...one to simultaneously complete an ordered set of scarless ligations following digestion with Type II restriction...

  20. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...
Showing: 281 - 300 of 534 results