We narrowed to 568 results for: CHI
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TypeBlog PostPublishedMay 30, 2023, 1:15 p.m.... of its biggest advantages. Specificity can be achieved using specialized promoters, Flp or Cre dependent...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...
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Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m.... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein...pulldown of a protein of interest, much like using ChIP for studying protein and DNA interactions. Unfortunately... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....including difficult-to-manipulate primary cells, achieving knockdown within 1-2 hr of reagent delivery. TRIM-Away... gene expression regimes that cannot be easily achieved with classical gene overexpression and knockout... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m.... making fluorescent protein fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent...Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom PH, Oler... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....approximately the length of your average PhD to achieve!) Each field has its own framework: a particular...images, and links to pay attention to. If you’re watching a video, you now have moving images, noises and... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....the University of Illinois and the University of Michigan - initially cancelled their plans, but have since...plans and budgets working around issues such as childcare using money from their increased salaries. In ... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....efficiency so that the greatest effect possible will be achieved. On the other hand, if you are trying to knock-in...PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI:10.1038/... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m.... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m.... or not proteins from the fission yeast, Schizosaccharomyces pombe, have the same function as their counterparts...yeast for young geneticists: A primer on the Schizosaccharomyces pombe model system. Genetics 201, 402-423... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....expression Generally speaking, one may want to achieve either inhibition or activation of neurons in a...Stoica et al., 2013). Vector delivery may also be achieved via injection into the liquor of the cerebral ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....inducible promoters, light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas...Mayo-Muñoz D, He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus (bovine), X. laevis (frog), D. rerio...submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m.... expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of...Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional Resrouces... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....relatively high efficiency. These alterations are achieved through DNA degradation, thanks to the unique .... Cas3 or Cas9? Large genomic deletions can be achieved using Cas9, but Cas3 may be a better option. To... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, ... Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T.,...