...you’ll want to ensure that your buffer components are compatible with your conjugate and conjugation chemistry...the assay. Where possible, it can be useful to compare recombinant antibodies to their parental hybridomas...majority of antibodies are still equally effective compared to those aliquots frozen immediately post-harvest...Chen E, Haidar JN, Srivastava A, Goldstein J. Comparison of predicted extinction coefficients of monoclonal...

...collaborations, and commitments to and proof of compliance with the grantor’s requirements as well as state...reports are submitted and project evaluations are completed. To enter and advance the profession, you can ...Professional Development Strategy to increase your competencies and skills in the grants profession. Your professional... and the candidate’s knowledge of the 8 core competencies through a multiple choice exam.  The bottom ...grant professionals and to build your skills and competencies. Becoming a grant professional is rewarding ...

...into a translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including...TAP) once referred specifically to a combo tag comprised of a calmodulin binding peptide (CBP), a TEV cleavage...ProtA IgG-binding domains. TAP has since come to encompass several other tag combinations, though frequently...and Gly residues   This article is not a comprehensive guide to all tags, but rather a quick overview...Biotechnology Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification...

...fields in order to provide useful solutions to complex challenges. As a cross-disciplinary researcher,...Many of the challenges in science today are too complex for any single discipline to solve them on their... disciplines leading the way, the future of complementing published research with video overviews was ...may never access online journals or even use a computer.  Currently, two of the more popular biology videos...derived from research. The platform uses video to complement traditional print journals and expand our ability...

... research compiled in the ELN. Without such references the record of research is incomplete, making it...commercialization office, the CIO’s office, and research computing. The two guiding principles emerging from the ...the record of research captured in the ELN is incomplete. Thus, to support reproducibility an ELN should...select and deposit individual documents or more complex datasets into the desired repository. They also...

...necessarily mean that you are a future employee of the company. It does, however, offer you an opportunity to ...like to work, get online and take a look at the company or institution’s career page, where you’re likely...openings. A good approach is to do this first for companies at which you’ve already made a personal connection...result in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying...development center, if one exists. Get online! Review company and institution career pages. Make yourself visible...

...the ability to cleave the RNA target.  Table 1: Comparison on Common CRISPR Enzymes Name  Enzymatic Domains...Amplified nucleotides are combined with Cas 13a in complex with crRNA targeting the sequence of interest and...multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single gRNA expression. Although... cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a ... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

...brainstorm session is to start with something completely unrelated to the issue and consider how it could...unique solutions to the same problem. If your lab/company/team is still working towards a culture of encouraging...just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone could fill out the...of which, when implemented, impacted the entire company. It is important to note that the person who submitted... is an incredibly innovative group. Our entire company benefits from their policy! Perfectionism When ...

...sgRNA targeting the genomic site of interest. We compared the pCas/pTargetF system with the updated pEcCas...system.   Advantages of the pEcCas/pEcgRNA system Compared with the original system, the pEcCas/pEcgRNA is...strategy for plasmid curing, providing users with a complete editing process. The MUCICAT system While the ...dehydrogenase expression that is 2.6 times higher when compared to the industrial strain. In our metabolic engineering... pQCascade) with 4 rounds of transformation to complete the N-acetylglucosamine strain library construction...

...applying to the specific company or lab. Take some time to research the lab or company via their papers and...place to state why you’re applying to the job, company, and/or lab, and how you feel connected to their...apply for [title of the position] at [name of the company/organization]. I am a [adjective that describes... are interested in working for this lab and/or company. Remember to proofread your cover letter, and ...

...in the targeted brain region after stimulation compared to controls without Chr2. c-Fos is a reliable ... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different...Inhibition of neuronal activation is a little more complicated since basal neuronal activity in your brain region...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...applying optogenetic tools derived from direct comparative analysis of microbial opsins." Nature methods...

...revolutionised the way we apprehend molecular complexes and is still a very useful tool.    Like a guitar... These issues can be overcome by choosing a complementary pair of fluorophores. To maximize the FRET signal...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian...Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...especially when you investigate unknown molecular complexes. But it is a parameter that you have to keep in...

... of the policy comprised of numerous objections and concerns. Perhaps least compelling of these were those...to individual researchers. Yet accurate and comprehensible organization of data is essential for efficient...numbers of figures such that many have multiple, complex panels. PLOS, itself, has not finalized its definition...collections and interpretation of data, publishing complete data, rather than just a single projection of ...

...such as cancer since mice better recapitulate the complex interactions between cancer cells, therapeutic ...Scientists can make a new line of flies in only 6 weeks compared to months to make a transgenic mouse line. Over...be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish (Danio rerio) Since...embryonic development studies as their embryos are completely transparent. Thus scientists can easily observe...model organisms, the C.elegans genome is also completely sequenced and over 60% of human genes have an...

...tough. Is the content accurate? Are we conveying complex ideas in the easiest way possible? Are the shots...Jennifer. I had finally found my footing with the company. I’d identified the ways to get the most out the...things like lighting, camera angle, and shot composition.  So back to the drawing board.  Cycle 3: A Return... and availability, a traditional solution was completely out of reach for Addgene.  So, onto research!...  Figure 4: The remote directing setup, complete with wireless video receiver, camera control, ...

...sword Microtubules are one of the most important components of the cytoskeleton. They provide cells with ...you’re interested in that other most-important component of the cytoskeleton – actin? Actin dynamics control...manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage of BcLOV4’s light-induced...powerful enough, what about altering the chemical composition of the membrane itself? The Baskin Lab previously...K. H., & Chow, B. Y. (2022). Designing Single-Component Optogenetic Membrane Recruitment Systems: The ...

...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science... research is about and what you think you can accomplish. No matter how many times I say this, candidates...specific questions and experiments. Nobody EVER complains that a seminar was too easy to understand. Make...detail. Finally, state how successfully completing the research will advance the field, create a...yourself to me by email or in person. If you feel compelled to repay me in some way, I encourage you to help...

...size, while SSC values depend on the structural complexity inside the cell or on its surface. Bear in mind...on the cell’s size, morphology, and structural complexity.   Height, Width, and Area Within FSC and...each coming with a different size and structural complexity. After several steps of tissue processing prior... for a single cell gate. However, make sure to compare H and A (or W and A).   Live/Dead Gating Having...cells stain positively for both markers. When comparing two markers simultaneously, we commonly describe...

...lots of hustle and bustle, but the lab was very competitive and not very communal. That didn’t suit my collaborative...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible.... of effort, intimidating use of discipline or competence procedures Threats to personal standing – including...atmosphere makes you successful. Collaborative Competitive Papers have multiple authors One author plus...choose a grad school or postdoc lab. Having a comparison can be very helpful in uncovering strengths and...

... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...yield, brightness and improved maturation time compared to similar orange fluorescent proteins. In their...more than 2-fold brighter signal from Antares as compared to Fluc in this model. The construct for Antares...transfer of energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by ...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...