We narrowed to 388 results for: HAL;

1. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some...

2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ... wild type  (Oveido et al., 2010). One of the challenges of studying bioelectricity is that it can be ...

3. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

4. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...molecules by conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867...

5. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

6. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ... M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH, Hall RA, Smith Y (2019) Ultrastructural localization ...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...

9. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...

10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

11. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...

12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

13. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...

15. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ...

16. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your...

17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

18. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

19. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...