Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 729 results for: NSI;

Showing: 281 - 300 of 729 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...number of building blocks with just two or three inexpensive enzymes. Now, a new toolkit - aptly named GreenGate...endonuclease of choice, BsaI, which can be very sensitive to temperature fluctuations. He says their lab...problem back to the endonuclease. “It’s just a sensitive protein,” he says. “There is nothing you can do...

  2. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... from other scientists doing interesting work. Consider this to be like any other research project. You...develop relationships, but if it is not possible consider a phone or video call. If you do decide to use...personal questions can elicit the most valuable insights you will get from the interaction. Most people...

  3. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...organism has already been made, and it would be expensive and time-consuming to repeat the process with ...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White...level necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates...

  4. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...partners, with the launch of antibodies in China and expansion of viral services available in South Korea.  ...addition, we released the MORF collection, a comprehensive transcription factor library, in lentiviral ..., Korea, Japan, India, and Brazil. Through an expansion of our export license application service, we’...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...a new and versatile binary Ti vector for both transient and stable gene expression applications in plants... (GUS) and GFP reporters, as well as multiple transient and transgenic systems, Sahoo et al. showed that... a novel versatile gene expression vector for transient assays as well as stable expression of foreign...

  6. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ..., yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald...multicolor labeling and FRET. Specialty constructs for biosensing. Over 300 plasmid backbones spanning a rainbow...color variants. The mEmerald plasmids offer a comprehensive list of readily available targets since the ...

  7. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...fluorescent proteins to observe what’s happening inside cells for at least a couple of decades. Green is... PA FPs with light of specific wavelength and intensity allows for optical labeling and tracking of proteins...capable of revealing, for example, how muscles work inside a living animal. For those making use of these ...

  8. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...for the user. This approach serves to maintain consistency with published screen interpretations and allows...curated CRISPR screen data are also available via comprehensive pre-compiled downloads and via a full-featured...are fully open access and available without any licensing restrictions.    As CRISPR technology continues...

  9. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...expanding your knowledge base extensively, you open doors to fresh insights and broaden your understanding...Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple of postdocs...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... these techniques and is a multi-step process consisting of digestion, purification, ligation, and transformation..., TOPO cloning, one step cloning, and overlap extension PCR. Although each type of cloning has its advantages...combine the products and digest with methylation sensitive restriction enzyme DpnI to remove the templates...

  11. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link...

  12. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...labs. Disposable plastic materials are fairly inexpensive in this country and we are quick to throw out... gel on a large glass slide using only surface tension.  It's a bit tricky to pour the right amount so...plastic and solder this thing together.  One of the downsides—there was no cover so you had to be careful not...

  13. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...a relatively pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super...lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for studying...

  14. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...cells. Until now, tools for doing this were labor-intensive and required using manipulations such as injection...sites in the construct to different lengths, the density of cells that are labeled can be altered to dense...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

  15. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...how Protein A and Protein B interact. You read extensive research on the two proteins and come up with ...antibody against Protein B. The antibodies are both extensively published, knockout validated, and work in your...with human antibody sequences.  Additional considerations Although isotype conversion is routinely used...

  16. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...proteins in vivo. These probes however can be expensive to make, hard to deliver into living systems, ..., and often do not fold properly once inside cells.  Developing the HA frankenbody To combat the issues...found that the HA frankenbody is effective in sensitive cells such as living neurons, and can be fused...

  17. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...markers and replication origins, but there is extensive heterogeneity in the feature sequences due to ...control elements in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to...reliable feature annotations. Even with these extensive efforts, such an algorithm has limitations. For...

  18. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...at our graphics and design editors. How about condensing a week’s worth of the world’s scientific discoveries...like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance of copy...in the science communications world, you might consider an internship or a short program to build skills...

  20. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press...
Showing: 281 - 300 of 729 results