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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 281 - 300 of 628 results

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...ahead of most companies in creating a welcoming, inclusive workplace for everyone. We had Unbiasing Training...movement. We are carefully attentive to genuine inclusion, but not worried because we are ahead of the curve...

  4. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...journal for formal peer review. Some organizations, including some journals, also support open peer review or...community significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in ...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

  6. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...pillars for antibody validation.  The 5 pillars include genetic strategies that test expression in knock-out...conditions. Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get...

  7. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing...terminus is favorable for tagging. However, if it is unclear which terminus should be tagged, the dTAG lentiviral...

  8. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide...

  9. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...photobleaching rate is not linearly related to power, the conclusions reached at high power may not translate to real...cells at a power used for live-cell imaging. To conclude, the most photostable fluorescent protein can ...

  10. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...

  11. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...associated with cellular damage and dysfunction, it is unclear what the precise mechanism behind the production...been documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. ...

  12. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...combinatorial expression of other genetic elements including transcription factors (Cheng et al., 2014) and... with effective gRNAs are publicly available, including those found at Addgene! Figure 1: Strategy...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly...

  14. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science...

  15. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...

  16. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... species of bacteria.  A note on anti-CRISPR nomenclature Acr family proteins are named for the type of...from pathogenic bacteria’s CRISPR-Cas system. Conclusion CRISPR is a great way to make those genome edits...

  18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ... infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and ...can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

  20. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...
Showing: 281 - 300 of 628 results