We narrowed to 552 results for: PPL;
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TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....problem for fusion proteins. One of the major applications of FPs is to visualize the localization, dynamics...An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein...
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Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue ... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....Persistent infection by adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136...on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral Vector Production and Troubleshooting... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....with an abundance of protocols and examples of applications from across disciplines now available. In this...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....PIPs, resulting in a fluorescent readout. Applications for bimolecular fluorescence complementation...resulting from the assayed PPI. This high-throughput application is much more straightforward to carry out because... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m.... primarily on fluorescent proteins and their applications, tackles much broader issues in this brief volume...successful scientists have outgoing personalities to supplement their intellectual prowess and scientific skills... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical methods for fundamental...fundamental scientific discovery and high-impact applications. Alvin Hsu is a graduate student at Harvard... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....notice typically should include the paper, any supplemental data, software repositories, or other information.... These variations are suited for different applications and should be carefully chosen for each group... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m.... critical for a number of popular scientific applications, and the quality of a standard curve can make...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95. Additional... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m.... and have been adapted for a wide variety of applications - as mutagenesis tools for genetic studies (...at one or multiple desired genomic loci. Many applications of these systems have already been demonstrated... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m.... to eliminate the use of antibiotics by their suppliers, and public health and medical organizations are...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....-target editing in a cell culture model. The application of Cpf1 to genome editing is exciting both in...in terms of basic science and translational applications. This discovery of this type V CRISPR system ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...the peer-review system of journals and grant applications, for example. However, at the same time, it'... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated knockdown...type or tissues; this is useful in gene therapy applications where a distinct subset of the cellular population... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m.... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, 073704 (2017). Hurt, R. C. et ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...https://doi.org/10.1126/science.aaq0180 Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....way to bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids...pestis using plasmid incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs...., Recent technological updates and clinical applications of induced pluripotent stem cells. Korean J ... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....connection you made at the job fair when you formally apply for the position. This post on Business Insider ...those relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....-strand breaks like those created by Cas12a. Applications of TnpB One of the major challenges in genome...viral vectors and for multiplexed gene editing applications. As research into TnpB continues to evolve, ...