We narrowed to 611 results for: REV;

...these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV...

... other fields or writing grant proposals to be reviewed by interdisciplinary panels.  And highly successful...Our 2018 workshop took place in Boston - you can revisit the activities of our attendees there online with...

...allowing for the targeting of sites that were previously unavailable. Figure 1: The PAM sequence...specific location, it must be accessible by Cas9. Our previous blog post The PAM Requirement and Expanding CRISPR...

...science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software development... the morning! 9:00 AM--Technical implementation review. The developers discuss which front-end and back-end...

...constructs could lower genome editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease...observed slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target...

... a DNA double strand break (DSB) in the genome wherever the user chooses. After the initial cut, the next..., Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...

...collaborator Some of these people you meet will review your papers or grants in the future, and don’t ... are talking to is really in your direct area.  Review the conference schedule and attendance list. Plan...

...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...

...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...induce genome editing. Such a system has been previously described for transcriptional activation/repression...

...The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell...

...combines these approaches to enable rapid and reversible recruitment of chromatin complexes to any genomic...regulation and subsequent mathematical modeling. Its reversibility allows precise and quantitative analysis of ...

... the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change...study, the Woolley lab showed that these photo-reversible protein-protein interactions can control subcellular...

...Berkeley at the time and an eLife ambassador, had previous experience organizing open access events and grassroots...Scientific Outreach at Code Ocean and through her previous work at Center for Open Science and Sense about...

...studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely that for breast cancer. What are tumor...malignant forms and noticed that myoepithelial cells prevented progression to malignancy, concluding that these...

...interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical signaling ...gradients for control of growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed...

..., see image above). We then select forward and reverse primers for each sample with the help of DNA-editing...prepared with the primer stocks for forward and reverse reactions, respectively, and then shipped along...

...detecting genome-wide double strand breaks (BLESS), revealed that these mutants do not cause off-target effects...to their enhanced specificity. Additional work revealed that mutations in the mobile Rec3 domain facilitate...

...Step 3: Reviewing the plasmid summary on the Fragmid website.   Step 3 is simply a review of the ...

...enter (and thus infect) cells, this safety feature prevents the virus from replicating and spreading in non-target...Types of Viruses Viral Vector Elements Tips for Preventing Viral Recombination  ...

...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A....number with links to the plasmid pages for your review. We’ll also mail you a deposit kit with instructions...