We narrowed to 343 results for: SEF

1. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...differentiate into multiple cell types makes them useful models for developmental processes and promising...

2. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   .... While the physical titer generated by qPCR is useful, I test a few different concentrations of virus...

3. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ...assembly reactions in which a single component was purposefully omitted. Since any assembly is dependent on ...

4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...editing experiments Prediction models only become useful once they find their way into the hands of users...

5. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...application. I found this step to be incredibly useful, as it gives a more comprehensive idea of that ...

6. Transferable Skills: Negotiation

   Type

   Blog Post

   Published

   March 3, 2022, 2 p.m.

   ...for opinions from a neutral third party can be a useful strategy to help get unstuck and move closer to...

7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ...These tools are available at Addgene and could be useful to labs wishing to create this kind of animal models...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...gRNA in a wide variety of mammalian cell lines. Useful for difficult to transfect cell types and can be...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...the dissociation of PIPs. Thus, whereas BiFC is useful in scenarios that call for a simple, yes-no answer...

10. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...Wu et al., 1997) inside of the capsid . This is useful for viral reproduction, and for causing contagious...

11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...in the real world, coordinates alone aren’t very useful. We’ll need something like Google Maps to help ...

12. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...

13. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...regardless of which is your favorite, colony PCR is a useful tool to have in your molecular biology tool kit...

14. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...data points generated by replicate values provides useful information about the accuracy of the curve, which...

15. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...subclasses and different species, nanobodies are useful for colocalization labeling of up to 3 targets ...

16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...experiences in graduate school. These skills will be useful anywhere I work in the future; they are transferrable...

17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...while using less resources and are particularly useful when trying to screen a limited number of cells...

18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression...

19. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    ...sensitivity of the assay. Where possible, it can be useful to compare recombinant antibodies to their parental...

20. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning. MXS Chaining...