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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 512 results for: SET

Showing: 281 - 300 of 512 results

  1. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...recommended pay scale – used by many institutions to set postdoc wages. The second event of note is a ruling...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...of several lasers simultaneously, can be used to set the laser output without manual manipulation of the...will open but will not be given an opportunity to “reset” and become receptive to light once again. Therefore...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...heterogeneous population of mutant cells, with each cell or set of cells containing a mutation in a different gene...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

  15. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657,...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP tags can be recognized by distinct...
Showing: 281 - 300 of 512 results