We narrowed to 852 results for: STI;

...number of extinct transposon sequences and then testing various combinations of changes. The basic functional...transposase system - one set of vectors for robust constitutive expression (pSBbi derivatives) and one set of...inducible expression (pSBtet derivatives). The constitutive and inducible expression vectors were developed...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...robustly express your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors...respond to blue light. We recently released a constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...

...experimental system comes with fewer ethical and logistical challenges than does working with animals. Because...staff from the ABRC are here to support you! From questions about growing conditions, to guiding students ... are available via e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach...science learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops, or staffing... staffing a booth at local science festivals, ABRC gets the word out about the importance of plant sciences...

...people, you will find the answer to your viability question. The miLab you see today is far different than... was viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...Although many emails resulted in no response, I was still able to get through to a number of experts, including...make sure you’re able to answer every possible question related to it. First impressions are everything...

...of the population as a whole, scientists gain statistical power in their observations. However, while flow... of FSC versus SSC that allows them to observe distinct cell populations and rule out dead cells and debris...complexity, these cell types will separate into distinct populations on a FSC versus SSC plot (Figure 2...flow cytometry experiment and will appear as a distinct population. After initially gating the lymphocytes...marker) to separate the B cell and T cell into distinct populations (Figure 2). An immunologist who studies...cells from a whole blood sample will separate into distinct populations of monocytes, lymphocytes and granulocytes...

...sure it is working? This article addresses these questions, which are critical in setting up a FRET-based... the wrong address in a different city, you can still develop a successful FRET-based assay since energy...interest: you just don’t know it yet! The poly-histidine tag often used for protein purification is a reliable...dye, as well as by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA ...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni2+...FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS one 8.5 (2013): e62739. PubMed...

...disorders. Antibodies administered as drugs are still immunogenic, meaning that they elicit an immune ...previous exposures to that specific antigen, pre-existing conditions, etc. that can vary widely between ...decisions about in vivo application and clinical testing. This would pave the way for improved patient outcomes...Burns-Naas, L. A. (2015). Chapter 27—Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis...Containing Unnatural Amino Acids: A Method Using Existing in silico Algorithm Infrastructure and a Vision...

...efficiency of multiplex gene editing. Moreover, existing multiplex strategies have been facing difficulties... fused base editors (nCas9-BE), as Cas9 is mechanistically more favored for efficient base editing. They...human cysteine tRNA stood out from their initial testing. It outperformed other tRNA candidates (either ...multiplex genome engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented... only minimal genetic payloads. Different from existing multiplex array architectures, including previous...

...Grammarly also offers basic writing suggestions, like alternative word suggestions. De-jargonizer The De-jargonizer... at these graphs when I need that extra push to stick with my writing routine. The graph of which days...detecting the readability of your writing. It makes suggestions on how to make your writing less complex. While...While I don’t think you should accept all of the suggestions Hemmngway makes (it wasn’t designed for technical...

... also be used to engineer new pathways with sophisticated gene expression controls. Most cell types do...two-component systems (TCSs). TCSs consist of a histidine kinase that phosphorylates a response regulator...domain from cyanobacteria to a common E. coli histidine kinase. This hybrid construct senses light, and...light; these two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...light-sensitive two-component system (TCS). A hybrid histidine kinase/photosensor senses various light inputs...

...when seeking your dream lab. The principal investigator's point of view If you are looking to join a ...course, the specific research field should be interesting; however, this should (in most cases) not be ...be the driving factor. The following questions can serve as a guide for the quest of a perfect "fit". ...Scientists". This post also includes a useful list of questions for a candidate to ask lab heads, lab members ...human disease. For more details and many more suggestions on planning for future success by finding and...

...or +1 (617)-ADDGENE (233-4363) with additional questions. Bacterial Strain Addgene can distribute your...Return to Article" button to return to the table listing all of the plasmids for this deposit. You can then...deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...hereby certify that I am affiliated with the institution ___. If this is no longer true, please modify...material under the terms deemed necessary by my institution or other third party rights holders. Once this...January 2016. It was updated by Angela Holmes and Christina Mork in February 2024.  Additional Inside Addgene...

...reach out to the program coordinator with your question(s). This precautionary step increases the likelihood...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...vast majority of programs tend to ask similar questions, thus devoting some time to writing a solid personal...interview. Formulate and practice a set of common questions with a peer or mentor. Several days prior to the...entry-level position following graduation. While these suggestions increase your competitive edge, they alone, are...

...Since there are many thousands of partitions, statistical modelling can be used to determine the actual...mismatch nuclease assays (2, 6, 7). Ability to distinguish between mono-allelic and bi-allelic mutations...digital PCR assays is that they are easily able to distinguish single-cell derived samples with mono-allelic...instructions on how to design such assays, in addition to testing their performance (2). Remember that although digital... digital PCR is a great screening tool, it still requires target DNA sequencing to confirm the exact nature...

...Biology. Can you tell me where to start?” is a question more and more students are asking. Teachers are...of educational programs all focused on current questions and experiments in the field of synthetic biology...from her own MIT curriculum. Drawing on current questions being asked in MIT’s research labs, BioBuilder... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

...antibody region that binds to the antigen) from existing antibodies. Each cleaved antibody will produce...(Muyldermans S., 2013). They have the unique distinction of being the only affinity reagent on this list...  To antibody or not to antibody?  That is the question… or is it? This post isn’t meant to convert anyone...trendy as that may sound. Validated antibodies are still an excellent option for many applications, and frankly...References Grumezescu, A. M. (2018). Drug targeting and stimuli sensitive drug delivery systems. Elsevier Science...

...researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter...SirHamhands, responding to a request for “any suggestion for CRISPR online course for beginners” on r/...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...!” — Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016,...

... the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments... to your cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose...like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature are compatible...It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap at least 24 bp...

...available to counteract the toxin. If, however, an investigator were to successfully clone an insert into the... (re-ligated empty vectors, for instance) would still express ccdB and therefore would die. This procedure...insertion will completely replace ccdB with the investigator’s insert of interest. Hence correct clones are... behind the “swapping out” of ccdB with the investigator’s gene of interest.   CcdB resistant E. ...further. Feel free to offer your comments and suggestions, and even to submit a guest blog entry of your...

...cytosine and adenine base editors that can be reconstituted in vivo to form a protein identical in sequence... plasmids and co-transfected into cells for reconstitution. Image from Levy et al., 2020 with permission...when paired with appropriate fluorescent optics. Existing red fluorescent voltage indicators, however, fall...neural circuit manipulations when combined with existing green-fluorescent GEVIs and blue-light-sensitive...The kit functions in the model cyanobacterium Synechocystis sp. PCC6803, and the fast growing strain Synchococcus...