...targets to measure! Let’s take a look at some of the hottest fluorescent biosensors new to Addgene this year...year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog is a new class of...smell the flowers - or rather, the lactate! The latest biosensor from Gary Yellen’s lab is LiLac, a fluorescence...fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato), they produce a temperature...over previous temperature indicators, it also demonstrates that taking advantage of fluorescence quenching...

... used to identify a gene mutation is the genetic test used for sickle cell anemia (2). Southern blotting...of restriction enzymes that cut DNA at specific sites (5). Imagine that you have a genome with unknown...cloning and analytical digests. A researcher can test the orientation of an insert in only a few hours...available although they are reported to have high error rates (10). It is projected that nanopore-based sequencing... C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia." New England Journal of ...

...recombination events, and the synthesis of alternative loxP sites to specify distinct patterns of recombination. In...Molecular and Cellular Biology, harnessed these attributes of Cre-lox to create the Brainbow mouse neural...defined using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions are mutually...be detected via immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine...doi: 10.1038/nrn2391. Epub 2008 Apr 30. PubMed. Intestinal crypt homeostasis results from neutral competition...

...with a red-shifted fluor. Here, NLuc internally excites the fluor through a process known as bioluminescent...exogenous excitation and with OgNLuc being the brightest since it had an emission near 600 nm – a clear...imaging. In all of these fusions coelenterazine intiates the fluorescent signal and these FP-RLuc8 fusions...see the movies demonstrating Ca2+ dynamics in cardiomyocytes. Enhanced Nano-Lantern constructs are available...bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence...

...there is variable transduction of oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons...Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 Microglia AAV9 > AAV2 & AAV6 Astrocytes AAV8 > AAV2...previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene ...

...curated their Ebola-related content. -And the latest updates on the ongoing outbreak from the CDC and WHO...Dallas was the first to be infected in the United States while caring for a patient, despite wearing protective... data on five strains of Ebola representing the latest outbreak and earlier ones, along with links to ...

...References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced...up in mice as poorer performance on forced swim tests and less interest in sweets. Further examination...be important to identify the key proteins and metabolites that are dynamically secreted by muscle during...

...discovery. With these screens, the investigator generates a cell population where all genes are knocked ...groups and, as a consequence, which genes are good candidates for being involved in the studied phenotype. ...These targeted screens can allow a researcher to test which of the differentially expressed genes found... is added), and decide which genes you’d like to test for phenotypic effects. Typically the phenotype ...interest (that were not found by Green Listed), and test if any of the alternative names works better. To...

...double-strand breaks (DSBs). DSB repair by HDR facilitates in-frame insertion of tag-encoding sequences ...assembling csRNPs in a 96-well plate format and tested parallel tag knock-in for 96 different genes in... negative control for the tagging experiment and test them in parallel so you have a good measure of ICC...clonal lines by single-cell deposition in 96-well plates: Characterize clonal lines by ICC and PCR genotyping...any cells positive for tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG...

...costume, and did their best to claim the costume contest trophy. We’ve had teams dressed up as plasmids,...table. This year, in addition to the Halloween contest, we’ve also carved pumpkins (yes, Blugene made ...temperature and humidity favors fungal growth. The ants bites down and locks their jaws into a leaf, and dies ...Addgene See some of our previous Halloween costume contests ...

... (see schematic below), enabling a researcher to test multiple tags for one protein quickly and easily...easily. These tagged plasmids also include cleavage sites should one need to remove the tag post-purification...post-translational modifications added to proteins in other eukaryotes better than E. coli. Plasmids designed for these... systems may include a signal peptide (SP) that routes the protein through the secretory pathway to improve...

...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage... grants too with pizza or pub.” The Cepko Lab celebrates any fellowships, jobs, or paper submissions with...dinner and champagne in the lab. The Church Lab celebrates successful moments in the lab with parties in...lots of interesting things from children, their classmates, and parents”. He also cooks and cleans with ...

...We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can...to work with a partner at IRCCS Besta in Milan to test small molecules targeting SMN1 and its partners ... to knockout, knockdown, or overexpress these candidates in a panel of ~30 patient derived peritoneal ...model systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling pathways for...

...recognized and operated as a nonprofit under the United States Internal Revenue Code 501(c)(3). That means we ...project we undertake and every service we provide is tested against fulfilling our mission. “Addgene’s nonprofit... post. We feel GREAT when you send us thank you notes and recognition. You are why we do what we do. ...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

...often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation...This workaround risks cellular phototoxicity and eliminates the possibility of long-term imaging studies....Overexpressed proteins also have the potential to form aggregates and may be toxic to the cell. Here comes the ...kinesin across microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels...

...meetings as opposed to phone calls most closely replicates how you’d interact with team members in person...helps everyone feel included in the project and accelerates the pace at which problems can be addressed and...the times that work best for them. This also accelerates the pace of research by circumventing the slower...techniques are being performed the same way at different sites. I did a lab exchange as part of the collaboration...

...complex, a model may be most effective. Once drug candidates are isolated, they can be validated for specificity...even be used to generate patient mutations and then test both individual and combinatorial drugs on for effectiveness...and validate the hit compound. The standard is to test the drug on models which are genetically proficient... can save time and money to make sure quality candidates are selected.      CRISPR aids in multiple...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula..., how to design good lesson plans, how to design test questions, and how to design lectures. I really ...happenstance. It was on Indeed or one of the job search sites. It's  a rare trek these days to go in cold turkey...

....  To develop HA frankenbody, the Stasevich lab tested several scFvs scaffolds previously proven to fold...frankenbody is that it remains bound to HA epitopes for minutes in living cells, meaning that scientists can track..., D. (1972). Localization of antibody-combining sites within the variable portions of heavy and light ... the National Academy of Sciences of the United States of America, 69(9), 2659–2662. PubMed PMID:4560694...

...(pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium... for imaging of small neuronal processes like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1...is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging GABA have been...pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger...