We narrowed to 746 results for: TRI

... and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv. PLoS...there are many scFv-based therapies in clinical trials or already in the clinic (Bates and Power., 2019... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

...development, and the shaping of the extracellular matrix. During immune responses, this same conformation...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...Addgene to accelerate biomedical research by distributing antibodies developed by IPI. Our integrin antibodies...

...type and laser voltage. The voltage describes an electric potential that can be applied to the photomultiplier...photomultiplier inside the cytometer to increase the electric current and thereby the signal strength. So, we...dimensions displayed in the plot. (B) A more restrictive gate results in fewer cells being considered ... personal preference, some people like to make stricter gates while some like to make looser ones (Figure...

...building and testing new products or methods, using metrics and feedback, taking a look at what works and what...partially reopened. But there were still many restrictions in place, most importantly, masks.  Besides ...Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The lab re-opened...very early in the pandemic with some incredibly strict safety protocols to keep Addgenies safe. Space ...

...This post was contributed by Damien Wilpitz founder and lead consutant at Experiment Designs Consulting...funding source. Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive...all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as many of these tools...they don’t have the money. If the department has a strict budget, or a lack of resources, then ask for suggestions...

...This post was contributed by guest blogger, Jessica Sacher, a microbiology PhD student at the University...abilities (6). This can be accomplished using a restriction enzyme with a short recognition sequence (like...probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of nitrocellulose membrane and...inhibitor cocktail. Line the bottom of 20 empty Petri dishes with thick filter paper and saturate each...

... genetics of Wilms Tumour, which is a type of pediatric kidney cancer. This was particularly interesting...company. This particular company carries out clinical trials of drugs and treatments for external companies....else. During the interviews, I asked if I could contribute more towards the scientific aspects of the company...grateful for some help.  I also get the chance to contribute content to the Addgene’s Website and travel to...

...AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst. While... 2013, Figure 1. Used under Creative Commons Attribution License. Within the nervous system, remember... 2019, Figure 4. Used under Creative Commons Attribution 4.0 International License.   Inducible promoters...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic...

...by chemical treatment or photobleaching or by stripping out the antibodies, then start over with the next...IF labeling of rat brain samples from the James Trimmer Lab, using recombinant antibodies with different... If you have questions or any favorite tips and tricks, leave a comment below. Happy imaging! References...Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched...

...and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant professor .... Figure 1: In the background are 504 restriction digests of PCR products used to identify a single..., which makes screening extremely rapid. This stringent selection allowed me to optimize a range of editing...these improvements from the Mello lab resulted in strikingly high editing efficiencies, obtaining as many ...

...parts in a specific orientation using Type IIS restriction enzymes (Weber et al., 2011). It can be performed...express recombinant proteins within the mitochondrial matrix.     Figure 1: A chart with MoClo kit recommendations...production of a protein of interest, and improving the nutrient profiles within given crops are all possible through...applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too. The AspFlex Kit ...

...This post was contributed by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience...series, I’m happy to share some of the tips and tricks and I’ve learned from numerous scientists at NIDA... to use optogenetics for? The technique is non-trivial; you don’t want to spend months doing surgeries...promoters and other genetic manipulations to help restrict expression to a specific neuronal subtype. However...

...favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together and delivered very ... was set very high from last year’s impressive entries, but our community of creative scientists delivered...

...Group, for these plasmids for the majority of the countries we ship to, first on a temporary and now on a ...information Edward Callaway   For researchers in countries with applicable export control exceptions for ...is accurate as of writing (Feb. 6, 2025), and we strive to keep our information updated, export controls...

...developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate...available from Addgene. Learn How to Analyze a Restriction Digest References 1. Henrici, R. C., Pecen, ... Miniprep Protocol Learn How to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources...

...covering the basics of science communication, tips and tricks for sharing your science with others, and introducing... sharing our educational resources and tips and tricks for those new to the lab, along with some behind-the-scenes...some much-deserved vacations, sunshine, and beach trips, of course.    *not me, though. I don't dance.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      ...

...provide resources to researchers in developing countries or that work in under-funded fields. Like Louis...among females in Ghana and in other developing countries” and “Almost all cervical cancers are caused by... our Cancer Pages Learn about Global Plasmid Distribution ...

...Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered...Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking for...

...lab discovered that cellular mismatch repair contributed to a large number of unintended prime editing...streamline the process of prime editing, the Liu lab contributed an improved prime editing “architecture” that...alternate approach to RNA stability This recent contribution by the Adamson lab also addresses the problem...doi.org/10.1038/s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

...pace. To submit, simply click "Contribute Data."    Figure 6: Contributing data to the Data Hub is easy...in the Addgene antibody collection to use and contribute to the Antibody Data Hub.   More resources on...