We narrowed to 697 results for: Tec;

...got to learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale...DeNatale and programming with our Chief Architect Pam McA' Nulty (check out our blog post on CRISPR software...presentation on the emerging CRISPR genome editing technology was a rousing success! Many of the students were...

... in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that are genetically...stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC-1a1 Pathway ...levels of kynurenine in the body, the brain is protected from stress-induced biochemical changes that could...

...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ... high spatial resolution are benefits of this technique. It is even possible to multiplex ultrasound dyes...

...blog post, keeping up with the newest CRISPR technologies and their applications can be exhausting. A ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong et. al (2013) Science paper). If you detect positive HR, isolate single-cell colonies, grow...editing, regulating and targeting genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt...

...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening...the validity of your biological model and the technical performance of your assay. But, in many discovery-oriented... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

...are also the foundation of multiple important technologies, including FRET and optogenetics. Even though...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and...protonated form of GFP is photoconverted, so this technique may not be appropriate for high-pH cellular compartments...

...career goals, and celebrate accomplishments. These techniques are laid out in Vincent et al., and are easily...a recap of accomplishments, from learning new techniques to finally figuring out a tough experiment, sets... in every lab, but the principles behind this technique are beneficial for researchers at all stages. ...

...inner beauty of familiar flowers." This new x-ray technique provides scientist with a new method to explore...by Darwin on his voyage. Previous destructive techniques were incompatible with the need to conserve these...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...

...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...found in our in-stock preps. If a request has technical considerations that may affect expected titers...

...to learn techniques from each other, access specialized equipment, or ensure that techniques are being...part of your collaboration. Luckily, there are techniques and tools that helped make my experience run ...

...environmental conditions, cells must be able to detect extracellular stimuli. One way they do so is through...pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance Energy Transfer...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and ...

...2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene....it is requested from Addgene many times as the technology disseminates throughout the research community...

...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty... thinking about, for example, how to use new technologies to reach the right audiences and how open access...

...the less acknowledged perks of scientific and technical training is that these educational paths prepare...encompasses millions of choices including pharma, tech transfer, management consulting, science communication...Small Company Big (e.g. Pharma) Small (e.g. Biotech) Develop expertise areas quickly Jack/Jane of...

...lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070...vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019...

...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

...more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers...pre-clinical research. However, standard terms and techniques are not enough to move the CRISPR field forward...

...Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...

...how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described.... These toehold switches can be used to quickly detect the presence of Zika virus. Read this post from...Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly barcode a set of ...

...present in prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...