Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
Type
Blog Post
Published
April 5, 2018, 12:42 p.m.
...promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product as a template... design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after editing, make...