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We narrowed to 707 results for: ang

Showing: 281 - 300 of 707 results

  1. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence within that region... region. We have designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers... difficult regions and send the plasmid off for Sanger sequencing.     If a repetitive region is present...

  2. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...). G. stearothermophilus grows at temperatures ranging from 30-74 °C, with an optimal growth temperature...or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity 35 - 45 °C 50 - 70 °C Genome...were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity is expressed as Kcleave...

  4. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...wanted to major in biochemistry. There, I had exchanged contact with Amy C., Addgene’s intern coordinator...into perspective just how much one’s environment changes them. Addgene gave me the chance to become a real...opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it ...

  5. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... of disciplines. Cast your net wider, explore tangential subjects, and let curiosity guide your intellectual...beyond your immediate field, fostering a diverse range of perspectives and sparking innovative thinking...embraces the unexpected and encourages exploration of tangents or seemingly unrelated ideas. It's beneficial ...

  6. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ... origin of replication (which controls the host range and copy number of the plasmid) and typically include...biologists to broadly utilize plasmids across a wide range of applications. Some common plasmid types include...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...

  7. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...conflicts are a common reason for scientists to change labs – do as much as you can to ensure a good fit...possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is a warning sign...advisors and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

  8. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...region may be changed.   Chimeric antibodies can help A chimeric antibody is made by changing an antibody...will react with the same secondary. If you were to change one of the antibody’s isotypes, however, you could...

  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...nice to have, not a need to have Must be ready to change projects frequently with little notice or choice...someone else who can do better, no grant writing Job changes can happen regularly (every 4-6 years is common... create interpersonal issues (one bad apple can change entire culture) Specific requirements for hiring...

  12. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...ahead of time included contacting YNP staff to arrange permits to visit places not accessible to the public... around these hot springs make them extremely dangerous, we used telescoping poles to collect water samples...night was fun but also very tiring. It feels a bit strange to be using a micropipette and gloves at a campsite...

  16. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided genome editing...expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing ...

  17. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...devotion to seeing your ideas become something tangible.  When you think of entrepreneurship in the 21st...company’s development  Don’t be afraid to email strangers asking to meet. I have cold emailed hundreds of...including Harvard/MIT faculty, venture capital and angel investors, and business development heads at major...

  18. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...past the chatting stage you will rarely make any change in your skill sets or activate the helpful accountability...of working with a mentor.The responsibility of arranging meetings and setting the direction in a mentoring...for Choosing a Good Mentor", and more! Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

  19. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...

  20. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ... tremendously complicated entities with a large range of characteristics (color, brightness, photostability...FPbase.org FPbase is a project written in Python & Django, using a popular database management system (PostgreSQL...users to see when a given page has unmoderated changes and to view previous versions.  Currently, all ...
Showing: 281 - 300 of 707 results