We narrowed to 287 results for: c rel

...a variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a ... lab, Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version...measurement of different RTKs. Although this sensor relies on a change in localization to the cell membrane...

... easy for a researcher to quickly generate N- and C-terminal epitope tag fusions to a protein of interest... was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, ... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...

... increased processivity and editing efficiency relative to PE2 (Doman et al., 2023), but this improvement...Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna, J...Figure 1: The first steps of prime editing. DNA release and heteroduplex resolution are not shown. Created...noncoding off-target landscapes for ABE8e and PAM-relaxed ABE8e-SpRY. Using deep‑learning models, the team...and false-negative (0–19%) rates. In clinically relevant HSPC models, Inrich-seq revealed numerous high-frequency...demonstrating durability, tunability, and clinical relevance. AIMers Deepak Shivalila described Wave Life Science...

...tuned for their viral preps coming soon! DeWeirdt, P. C., et al. (2022). Accounting for small variations in...in the Fei Chen Lab, relies on the vault ribonucleoprotein complex: a large, barrel-shaped structure of...oxidation or protocols for tissue clearing and correlative light and EM imaging. Figure 1: SIM images...AAV5 packaging plasmids at Addgene By Meghan Rego Reliably achieving high yields for your viral vector preps...ability to recognize their target and not closely related proteins in cells (Figure 6).  Figure 6...

...repository 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation  1:18:45 -...45), there are also initiatives to create more reliable reagents for future research. I’ve talked to many...many researchers who have complaints about the unreliability of antibodies. IPI is working to address this...repeat previously published results. This over-reliance on the perfect story for a “high impact” publication...ways to publicise negative data - we don’t have to rely on traditional journals. There’s social media - ...publishing community views reproducibility, how industry relies on reproducibility, and how you can push for more...

...composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase. CalFlux...Many intracellular sensors of events like calcium release have been made using fluorescent resonance energy...biosensors, the intensity of the FRET signal can be correlated to specific concentrations of biological molecules...et al. (7) wished to trigger intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring...

... Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse C, Kubo M, Hiwatashi Y, Goshima G (2012) An Inducible... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi...2018) and the model bryophyte Physcomitrium (Physcomitrella) patens (Bezanilla et al., 2005; Nakaoka et...Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens with an Internal Marker for Silencing...Inducible RNA Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast...