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Showing: 281 - 300 of 523 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

  4. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...

  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...not yet fully understood, so it is important to carefully select the target locations of knock-in studies...systems offer a number of different options. By carefully considering your ncRNA of interest, you should...

  9. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...for follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team...Sciences at Arizona State University. His group carries out research in synthetic biology, nanomaterials...

  10. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...the molecular biology to learning how to grow and care for the worms. As an example of one of the problems...cheap using equipment that would otherwise be discarded. For instance, the centrifuge in the picture had...

  11. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...15%, 25%, 40%, and 60% iodixanol solutions are carefully layered and then the viral suspension generated...digested to remove any residual AAV plasmid that was carried over from the AAV production process. Either SYBR...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...this protein compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated...with this encapsulated enzyme produced 20% more D-glucaric acid, suggesting that MPyV system can improve ...

  14. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1: Left: recombined...and adopts an open conformation in the absence of cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org...

  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...can be transfected with your transfer plasmid, carrying your gene of interest, and the two helper plasmids...undergoing titering, to remove residual plasmid DNA carried over from the production process. For more details...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of ligations in an hour or less...one to simultaneously complete an ordered set of scarless ligations following digestion with Type II restriction...

  17. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling... 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA... Contransfect the PITCh vector with the vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step...

  19. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to the light, where they appear...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

  20. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it is expensive...marker can be removed by Cre-mediated excision for scarless tag insertion.   In addition to the obvious advantages...
Showing: 281 - 300 of 523 results