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...larger. And since the library contains many plasmids, care must be taken to ensure that all of these plasmids...powerful research tool, but their proper use requires careful amplification and maintenance of good representation...

...decolorized as gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding...resources.   Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html...

...institute where their typical workdays will be carried out until the end of the appointment. The visiting...don’t forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it...

...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

...intensity and/or duration. Both of these strategies carry the risk of off-target effects. Harald Janovjak ...of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles...lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to ...

...well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical copy on hand as... to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing a Lab...

... be assembled in a unique order with no cloning scars. The Elledge Lab showed that a five-piece assembly...researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...

...regions of the brain. However, the Cre-dependent AAV cargo would only become activated in the presence of Cre...design and interpretation of results. Take extra care when evaluating how this serotype works in your ...

...the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I acts as a monomer...

... disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in ...is abnormally deregulated in TS and can lead to cardiac developmental defects. Eight genes from the X chromosome...

...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

...early career scientists using fluorescent proteins in their research. These awards help early career researchers...

...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

...ago (Field et al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys. Surprisingly...But our data does highlight the importance of a careful characterization of transgene expression. Which...

...used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...

...for follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team...Sciences at Arizona State University. His group carries out research in synthetic biology, nanomaterials...

...not yet fully understood, so it is important to carefully select the target locations of knock-in studies...systems offer a number of different options. By carefully considering your ncRNA of interest, you should...

...the molecular biology to learning how to grow and care for the worms. As an example of one of the problems...cheap using equipment that would otherwise be discarded. For instance, the centrifuge in the picture had...

...the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling... 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations...