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We narrowed to 546 results for: epo

Showing: 281 - 300 of 546 results
  1. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...antibodies expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the... VH and VL sequence. These plasmids have been deposited with Addgene for distribution to the scientific...
  2. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...
  3. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    ...Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding function to... (with rHUH). Many of these tools are already deposited at Addgene in multiple formats: for use in different...
  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based...
  5. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence...
  6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators...
  7. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...open science, Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations...
  8. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...the development of a vast number expression and reporter plasmids and the free exchange of these vectors...characterization of NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol...
  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...
  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...they are integrating viruses – they physically deposit their cargo into the host’s DNA. These viruses ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just...
  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo...
  12. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...the experiments performed by our thousands of depositors. That means their original articles are your ... Addgene Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our...
  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...
  14. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...supervisor might expect weekly or biweekly progress reports. However, a hands-off busy supervisor might get...update, and whether he or she would like a written report or to meet in person.   Regardless of your supervisor...
  15. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...
Showing: 281 - 300 of 546 results