Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 294 results for: expression vector

Showing: 281 - 294 of 294 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... multiple parts transcribed together. They can express one or more gene(s), and typically begin with a...UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly of multiple Level 0 parts into...

  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...drawback of CRISPR-Cas9 technology is that most expression systems are constantly “on”. There is no mechanism....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR...GEVIs. Some constructs are also available as viral vectors (here and here).  Figure 3: JEDI-2P was identified.... By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence...

  6. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...along with Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal...on the Addgene Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian...

  7. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong "Tom" DuanVisualizing...

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...continues to be challenging. Organism specific viral vector systems would also be beneficial for many systems...snail can be manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... whatever molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand! A...better detect dim structures or low levels of expression with the same imaging workflows you already use...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility...

  10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...with natural chain termination at stop codons, expression levels of these tagged proteins are often very...could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA ...intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA guide is typically...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...

  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...MK, Elfinger M, Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...

  13. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...for many molecular biology protocols, including vector-insert joining for recombinant plasmid construction..."Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog...Barany, Francis, and David H. Gelfand. "Cloning, overexpression and nucleotide sequence of a thermostable DNA...: PMC146030. 26. Nishida, Hirokazu, et al. "Overexpression, purification and crystallization of an archaeal...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    .... PubMed Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...
Showing: 281 - 294 of 294 results