We narrowed to 872 results for: fer

...proteins that confer affinity for a target – and this serotype can be selected to preferentially target your...voltage indicators reviewed.   References and Resources   References Beck C, Zhang D, Gong Y. Enhanced... These types of sensors can have a variety of different components, but all contain a FRET pair, a genetic... genetically encoded component of the system to offer unique advantages depending on your experimental...powerful tool that swaps capsid proteins from different viruses to optimize them for targeting your cell...

...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...antibodies, context is key.    References and Resources References Evan GI, Lewis GK, Ramsay G, Bishop... or the C-terminus, Evan, et al. isolated six different monoclonal antibodies (Evan, et al. 1985). Three... found that the N-terminal half of the antigen differs significantly between human and the other species...-9E10 variable regions and sticking them onto different scaffolds. But over the years, several alternative...

...library protocols you can use as a reference. Table 1: Examples of Different Pooled Libraries at Addgene.  ... plasmids in your library (Are the inserts of different sizes?), and the desired coverage for the library...For this reason, if your library has inserts of different sizes (such as a transcription factor overexpression...your growth time (e.g., 16-18 hours). Finding reference protocols There are many decisions to make when...pDNA Library Amplification protocol are useful references. Skim through our Pooled Library page to find...“one-size-fits-all” protocol! Each library is different, and you should always experimentally test the...

...variants—nuclease-competent or nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge... both cutting and regulation of expression at different genetic loci within a single cell. One might imagine...use of “orthogonal” Cas9 proteins derived from different bacterial species, each of which interacts with... (1). Although this is possible, this strategy suffers from several limitations. Perhaps most importantly...expression (6). Altering the length of gRNAs targeting different sites of interest thus represents a straightforward...introduced together with truncated gRNAs targeting a different set of loci to induce simultaneous cutting and...DNA sequence or regulating gene expression.     References 1. Esvelt, Kevin M., et al. "Orthogonal Cas9 ...

...Labguru, Quartzy offers software designed with scientists workflow in mind and can offer a one-stop solution...create lists of your favorite protocols for future reference. The exchange currently boasts over 2,500 protocols... is a subscription-based software, Labguru does offer free trials and pricing options for academic labs... limited to within your lab group, Labguru does offer access to protcols from external vendors, such as...orders. Quartzy caters to life science labs and offers the service free of charge to scientists by allowing...101 articles on various plasmid features, and reference tables (i.e. antibiotic concentrations, commonly...

...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...non-linear and you should use a different method of analysis. Different fields and assays have varying ...the assay is run, ideally with freshly prepared buffer. This is because standard curves vary depending...range covers a wider span of concentrations but suffers from decreased specificity, especially as it approaches...

...antibodies directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using...downstream step, you’ll want to ensure that your buffer components are compatible with your conjugate and...you’ve collected your antibody in the appropriate buffer, it’s time to quantify it. There are several options...our Antibodies 101 blog series.  Resources and references More resources on the Addgene blog Introduction...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

...Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring...requires careful selection of FP fragments and, preferably, some information about the structures of the...level, stability, and subcellular localization may differ from those of your BiFC construct, making it difficult...., do two proteins interact or not? — FRET is preferable when the temporal dynamics of PPIs are the focus...about science whenever he's not off the grid.   References Bischof J, Duffraisse M, Furger E, Ajuria L, ...plasmids and resources Visit our Molecular Biology Reference ...

...decorative" title="Check icon" %}   References and Resources References Hwang, J. M., Piccinini, T. E., Lammel...belongs to the penicillin family of antibiotics. It interferes with cell wall synthesis as its mode of action...beta-lactamase, just like amp is. Thus, the AmpR gene confers resistance to both carb and amp, due to their common...amp), and for some applications, the benefit it offers is marginal. Fortunately, amp can easily be replaced...phosphorylation of kan, which inactivates it. The NPTII gene confers resistance to several aminoglycosides, including...minutes). Pros: Widely available, cost-effective, confers mammalian resistance to G418. Con: Slow transformation...

...created with the same backbone but that contain different inserts. When you design primers to anneal outside...primer pair to screen for the presence of many different inserts. The downside: this type of primer does... The type of primers you use depends on your preferences. Either way, make sure to test your colony PCR...a standard PCR thermocycling program. One key difference is the plasmid DNA must be released from the ...and without an insert. These controls are quick references you can use when you run your PCR products out... an appropriate molecular weight standard for reference and to add a loading dye with glycerol to your...products have any mutations.  There are lots of different cloning strategies, but regardless of which is...

...PAM sequence requirements. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both...endonuclease domain, indicating that Cpf1 functions differently. Since Cpf1 loci are widely distributed across...could be adapted for genome editing. The use of a different nuclease could potentially overcome some of Cas9...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...shown the diversity of CRISPR-based systems. References 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...

...Cary Valley, and Holly McQueary References and Resources References Niedringhaus, T. P., Milanova, D....identify the bases (and, if necessary, separate different samples), the resulting data, or reads, are essentially...plasmid sequence. However, if there are major differences from the expected sequence, the QC team can manually...theoretical sequences submitted to Addgene have minor differences due to in silico variations and lack of verification...script for manually inputting known innocuous differences. This script compares the sequence for a 100%...Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis ...

...proteins also contribute to this regulation in different ways: methylating newly synthesized DNA, blocking...numbers can allow RepA to link iterons from two different plasmids, forming a pair of “handcuffs” that blocks...RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing," which...Plasmids under stringent control of replication may differ in the specialized proteins or sequences necessary...was updated by Emily P. Bentley in June 2024.  References and resources Further Reading Quiñones-Valles...Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number...molecular biology help with our Molecular Biology Reference Pages   ...

...best with diverse teams, to leverage the preferences of different personality types, and to devise strategies...biotech clients. In this realm, there are a few different kinds of challenges that consultants get called...consulting experiences, educational training, and conferences, and synthesizing this knowledge into a coherent...excessively demanding. For instance, a client may offer a lot of pointed feedback or abruptly change needs...consulting firms coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs...prepare you for a successful career in several different fields. While some people continue to enjoy the...

...example, CRISPR activation has been used to differentiate induced pluripotent stem cells (Chavez et al...neuronal cells by activating genes that cause differentiation and to reverse HIV viral latency (Bialek et...directed towards that gene as there can be a large difference in guide potency. Thus, in cases where users ...cannot be used to study in cis regulation and also suffer from an inability to easily deliver the appropriate...Chavez et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post...DNA sequence or regulating gene expression.     References Behan FM, Iorio F, Picco G, Gonçalves E, Beaver...s41586-019-1103-9 Bialek JK, Dunay GA, Voges M, Schäfer C, Spohn M, Stucka R, Hauber J, Lange UC (2016)...

...today. It is now known that nearly 500 cell lines suffer from contamination with HeLa being the most frequent...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...the cell lines deposited with them and used by different researchers are free of contamination and match...from this bank. I am often asked, “What is the difference between materials available from commercial repositories...the same cell line from a colleague?”. The main difference is the QC and validation carried out by the commercial...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate... drug testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal...

...preparative experiments.  We’re excited to begin offering this service to further contribute to our mission...and discovery.” Here are the details. Plasmids offered as cloning grade DNA As we begin testing out this...that this service will be helpful to the many different scientific communities using plasmid tools. Details... We will ship you  2 µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such... our Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to ...other beta projects Read our Molecular Biology Reference ...

...fragments that are very different.” Because the fragments produced will have different overhangs, they can...enzymes. Now, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working...systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann...Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target...available, making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate ...

...purified protein at different acid concentrations. Overviews of the pKa values of different fluorescent protein... GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant was made by introducing ...reduces its size. The substantial size of GFP may interfere with the activity or the localization of the fusion... Another solution to avoid autofluorescence is offered by bioluminescent proteins. These probes generate...C-terminus of another protein. This may, however, interfere with the function of the protein. This is clearly...can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al. "Partitioning ...

...similarities with humans. However, rats have some key differences in physiology, intelligence, and human correspondence... generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed several...number of offspring (produces many seeds), self fertilizing, a small yet highly characterized genome, and...include understanding of allelic variation between different plant species and metabolic properties affecting... collections to help start your experiments! References: Blount, Z. D. (2015). The unexhausted potential...Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics...Plasmids Collection Page Read our molecular biology reference Find plasmids for your research ...