We narrowed to 823 results for: lars

...polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. PMID: 1333042. PubMed Central... thought that poly(A)s are used to control the cellular concentration of regulatory RNAs and may additionally...secondary structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes...terminate transcription. Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas...Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297. PubMed Central ...

...Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...blogger Derek Simon. The surgeries and standard molecular neuroscience validation experiments we discussed...cemented to the animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber... Gi/o protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry...PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless...

...already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the Cas9 gene...compared to other viral vectors, AdVs can provide a large transgene packaging capacity. For instance, with...non-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding TALENs... B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most... animals in order to create animal models for particular cancer. That’s exactly what the lab of Andrea...

... that can bind effectively to protein targets, similar to how antibodies do. The structure of the common...(~150 kDa) and Y-shaped because it contains two large heavy chains and two small light chains that stick...antibodies to get to, like ones that are hidden in the molecular crevices proteins on the cell surface. Figure...optimally binding sybody, you want to start out with a large and diverse pool of sybodies that hypothetically...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148. https://doi.org...

...into embryos and then become larvae. There are three larval stages where larvae molt in order to continue...continue growing in the next larval stage. After the third larval stage, Drosophila will pupate, which takes...fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it...

...cells are a great option for viral production, particularly for labs that already do mammalian cell culture...for the generation of viruses, though they are a popular choice for protein production. The most commonly...infect insects. This is what makes insect cells a popular choice for protein production. AAV and lentiviruses...systems. Baculovirus transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect...in between passages to eliminate dead cells and cellular debris. While adding time (about three to four...supernatant in a centrifuge to remove any unwanted cellular debris, as you would when collecting a lentivirus...

...specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these...nucleotide sequences are exchanged between two similar or identical molecules of DNA. Gene targeting takes...targeting constructs. In the example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the ... the cells that have randomly integrated all or large portions of the plasmid. Deliver your construct ...insert GFP into the genome, we’d create a plasmid similar to the one shown below, where the sequence of GFP...CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult. Addgene depositors...

... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase...strains, including HB101 and derivatives, release large amounts of carbohydrates that can inhibit the cell...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...choice of strain will ultimately depend on the particular features of your plasmid, it is advisable to ...coli strains. Increase the volume Starting with a larger culture volume can improve plasmid yield. Our lab...gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around 220 RPM,...

...depositing lab. There may be other plasmids with similar names in the collection, but you can use this name...some of Addgene’s plasmids is an indication of popularity. A yellow flame indicates that a plasmid has ... on our website, one of the available filters popularity (e.g. only showing those plasmids which have ... than 100 times).     I guess it's cool to be popular, but how do I know if a plasmid is actually useful...If the depositor has indicated that there is a particular mutation found within the gene/insert, this information...means you can see which labs have already used a particular plasmid, and which experimental systems it was...

...role of individual genes or entire pathways in a particular disease process. While CRISPR is certainly not... mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily scaled up for...fluorescence-based cell sorting and screened for a particular phenotype. For example, mutant cells can be used...cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid...targeting the same gene can be strong evidence that a particular gene is actually important for a given phenotype...Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002/cpmb.8 ...

...results. The default setting is “Optimize for Highly similar sequences (megablast)”, which is very fast and ...often unreliable, we suggest using the “Somewhat similar sequences (blastn)” program for blastn so that ...your sequencing trace file. Unlike megablast, the regular blastn program uses a smaller word size and lower...protein sequences (nr)” database as it contains the largest number of protein sequences. Just below the BLAST...options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often have repeated...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition...

...transistor-like digital genetic switches that enable cellular computing. These logic gates built from transcriptors...of promises to not assert property rights for particular engineered genetic sequences; but it’s only the...computer languages are free to use. Of the 15 most popular computer programming languages, 12 out of 15 are...are now available exist within the context of the larger bioeconomy, and that it’s been 40 years since the... on the domestic market generates $300 billion dollars every year. That's two percent of the U.S. gross... we anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward...

...information while using less resources and are particularly useful when trying to screen a limited number... libraries have been shown to outperform other popular CRISPR libraries (see below). Let’s take a look...Brunello library was more effective than other popular CRISPR libraries in the depletion of essential ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...about the advantages of pooled CRISPR libraries for large-scale functional screens Read this blog post about...

... Boyden Lab, ExM has been steadily growing in popularity with an abundance of protocols and examples of...not something you find in the average cell and molecular biology lab, and EM is limited in the number of...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid..., Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-fold Robust... Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures using expansion microscopy (U-...Wijesekara P, et al (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat...

...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous...fortunate enough to apprentice with top scientists to larger groups of trainees at an earlier point in their...protein with easily measured activity, MDH is particularly amenable to in-class labs. Provost ultimately... at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of my students published...and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323. Knutson, K.,...dehydrogenase as a research model. Biochemistry and Molecular Biology Education. 38(5):324-329. Kowalski, J....

...specializes in developing novel technologies to analyze molecular mechanisms of genome functions such as epigenetic...transcription and epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions, ...isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed the...merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred requests, and...been very difficult for us to respond to such a large number of requests by ourselves without Addgene.... experiences in research labs. What are some similarities and differences you have observed as you have...

... the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...fluorescent proteins have become a mainstay in molecular biology. Scientists can easily utilize GFP-containing...biosensors has been designed to detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations...can use light to detect, measure, and control molecular signals, cells, and groups of cells in order to...applications for GFP. It has also been used to identify particular cell populations in drug screens, to visualize...strand break repair, and to label pathogenic intracellular microbes to visualize host/pathogen interactions...

...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was produced.   ...characterizes the sequence and abundance of each particular indel.   For a complete guide to ICE analysis...Typically, a yellow check mark indicates that a particular parameter was automatically adjusted to generate...+F or Command+F functionality to search for a particular sequence or name. You can perform more in-depth...Contributions, Indel Distribution and Traces - provides particular details about the indel profile of the edited...

...Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular Biology 432:1602... consolidated and centralized a collection of molecular biology tools so that both veteran and new Rett...severity. This exciting finding injected hope that a similar reversal may be possible for children and adults...RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and promising results for...Apparent Toxicity after Intracisternal Delivery. Molecular Therapy - Methods & Clinical Development 5:106...

...deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes...Kenkel.  CRISPR genome editing has quickly become a popular system for in vitro and germline genome editing...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus... with split AAVs. In general split AAVs break a large transgene in two pieces and package each piece into...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...