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We narrowed to 811 results for: lars

Showing: 281 - 300 of 811 results

  1. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ... Boyden Lab, ExM has been steadily growing in popularity with an abundance of protocols and examples of...not something you find in the average cell and molecular biology lab, and EM is limited in the number of...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid..., Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-fold Robust... Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures using expansion microscopy (U-...Wijesekara P, et al (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous...fortunate enough to apprentice with top scientists to larger groups of trainees at an earlier point in their...protein with easily measured activity, MDH is particularly amenable to in-class labs. Provost ultimately... at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of my students published...and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323. Knutson, K.,...dehydrogenase as a research model. Biochemistry and Molecular Biology Education. 38(5):324-329. Kowalski, J....

  3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...information while using less resources and are particularly useful when trying to screen a limited number... libraries have been shown to outperform other popular CRISPR libraries (see below). Let’s take a look...Brunello library was more effective than other popular CRISPR libraries in the depletion of essential ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...about the advantages of pooled CRISPR libraries for large-scale functional screens Read this blog post about...

  4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...specializes in developing novel technologies to analyze molecular mechanisms of genome functions such as epigenetic...transcription and epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions, ...isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed the...merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred requests, and...been very difficult for us to respond to such a large number of requests by ourselves without Addgene.... experiences in research labs. What are some similarities and differences you have observed as you have...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...fluorescent proteins have become a mainstay in molecular biology. Scientists can easily utilize GFP-containing...biosensors has been designed to detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations...can use light to detect, measure, and control molecular signals, cells, and groups of cells in order to...applications for GFP. It has also been used to identify particular cell populations in drug screens, to visualize...strand break repair, and to label pathogenic intracellular microbes to visualize host/pathogen interactions...

  6. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was produced.   ...characterizes the sequence and abundance of each particular indel.   For a complete guide to ICE analysis...Typically, a yellow check mark indicates that a particular parameter was automatically adjusted to generate...+F or Command+F functionality to search for a particular sequence or name. You can perform more in-depth...Contributions, Indel Distribution and Traces - provides particular details about the indel profile of the edited...

  7. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular Biology 432:1602... consolidated and centralized a collection of molecular biology tools so that both veteran and new Rett...severity. This exciting finding injected hope that a similar reversal may be possible for children and adults...RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and promising results for...Apparent Toxicity after Intracisternal Delivery. Molecular Therapy - Methods & Clinical Development 5:106...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...deliver larger cargo such as base editors and prime editors. While using AAV to deliver large transgenes...Kenkel.  CRISPR genome editing has quickly become a popular system for in vitro and germline genome editing...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus... with split AAVs. In general split AAVs break a large transgene in two pieces and package each piece into...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting and amplifying...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...advantages of oligonucleotides over proteins is their modularity, which is lost as soon as the aptamer is bound... Assays that require recognition of ions or particularly small molecules that do not generate an immune...Central PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with Nucleic Acid Aptamers.” Curr Med ...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ..., to search the literature, and to synthesize a large volume of information: basically, a scientist’s ...colleague, or your adviser was invited to write on a particular topic. Alternatively, maybe you’re researching...helps you later on to remember where you read particular studies or experiments. Later, you can easily... searched for recent research papers on these particular themes, for seminal papers on these themes, and...also did not limit myself to the well-cited or popular papers, but looked for papers from a wide range...present it all in one place to get a sense of the similarities and differences among proteins in the class....

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects... were produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus...intact SARS-CoV-2 virus. Scientists have been using similar pseudotyping methods with other viruses in a BSL...envelope protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric...in Human Cells Are Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe...nervous system after peripheral delivery. Human Molecular Genetics 10:2109–2121 . https://doi.org/10.1093...

  12. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...your science. While Reddit isn’t for everyone, particularly if you’re more interested in selling a product...topic”) which are essentially lists of forums on particular topics. Volunteer moderators set the rules for... and, at least sometimes, civil. r/Science in particular hosts a variety of conversations with people ...can ask designated scientists anything about a particular topic. Given Joanne’s expertise in science careers...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...and its family members serve as the cellular receptors for vesicular stomatitis virus.” Proceedings of ... can persist undetected; unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma... medium. Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource...Detection of mycoplasma contaminations.” Methods in Molecular Biology 946 (2013). 1-13. Pubmed PMID: 23179822...lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14. Pubmed PMID...

  14. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced...protein to study its function is one of the most popular applications of fluorescent proteins. These fusion...Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...291.5512 (2001): 2408-2411. PubMed PMID: 11264536. Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions...secondary antibodies on hand to mix and match with a larger collection of primary antibodies. This strategy...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...sections of papers that have successfully used your particular antibodies and refer to the manufacturer’s recommendations...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...Performing Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https:...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... target to specific subcellular regions, laser ablation can damage other cellular structures, and genetic...activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength...the flip of a switch. You may be familiar with popular light-sensitive ion channels used to control activities...Opto-RhoGEFs to study cell junction integrity in the vascular barrier of the endothelium, but they could be ...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...lymphocyte population distinct from cellular debris and other larger cells. Note, we don’t mind the warning... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...

  18. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh where he... - 7:11 : Introduction - Ghanaian scientist's molecular biology needs and why Kwabena applied for the ...Ghana after his PhD and the need to de-mystify molecular biology. 25:51 - 30:56 : Further biology work ...current resources to develop microbiology and molecular biology research programs. 30:57 - 40:16 : Kwabena's...Microbiology Pages Find Synthetc Biology Plasmids Find Molecular Biology Resources ...

  19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging...the epigenome may make certain processes, like cellular reprogramming of iPSCs, more efficient. In general...genes of previously uncharacterized enhancers. The popular TALEN-based LITE system, which uses light to regulate...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9... use. If you want to study the effects of one particular modification for which a targeted editor, like...

  20. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...remains in the cytoplasm. Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor...Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying the bacterial cell ...cell cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete ...CRISPR3 module of Streptococcus thermophilus (the popular Streptococcus pyogenes system does not work well...
Showing: 281 - 300 of 811 results