We narrowed to 632 results for: ncl

...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

... DNA there, although other restriction sites (including other XbaI sites not preceded by GA or followed...either be difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes...

...intact β-galactosidase produces pigment from x-gal (included in the transformation plate medium), turning the... colony blue. We should note that IPTG is also included in the medium to ensure transcription of the lac...

...photobleaching rate is not linearly related to power, the conclusions reached at high power may not translate to real...cells at a power used for live-cell imaging. To conclude, the most photostable fluorescent protein can ...

...distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest, increasing...terminus is favorable for tagging. However, if it is unclear which terminus should be tagged, the dTAG lentiviral...

...proteins that are found in and on everything, including your skin. Here are some general tips for working...degrade RNA. Keep a clean work area, which may include spraying your bench down with a product to get ...

...recognized for its biotechnological applications including conversion of CO2 into water and electrical energy... in cyanobacteria. This kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable...

...journal for formal peer review. Some organizations, including some journals, also support open peer review or...community significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in ...

...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

...pillars for antibody validation.  The 5 pillars include genetic strategies that test expression in knock-out...conditions. Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get...

...proteins potently inhibit type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017... the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP...

...ahead of most companies in creating a welcoming, inclusive workplace for everyone. We had Unbiasing Training...movement. We are carefully attentive to genuine inclusion, but not worried because we are ahead of the curve...

...other required parameters of the coupling system include: Wide fusion-tolerance — Click Biology tags should...HaloTag Collection of research tools from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands...

...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide...

...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...

...associated with cellular damage and dysfunction, it is unclear what the precise mechanism behind the production...been documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. ...

...combinatorial expression of other genetic elements including transcription factors (Cheng et al., 2014) and... with effective gRNAs are publicly available, including those found at Addgene! Figure 1: Strategy...

...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly...

...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science...

...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...