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We narrowed to 792 results for: por

Showing: 281 - 300 of 792 results

  1. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...Improvement of Cowpea, a knowledge database for this important agricultural plant. Her current research focuses...proteins in order to manage insect pests and to support sustainable agriculture. For this project the expression...in Mexico. At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

  2. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...make sure everyone in the meeting has an equal opportunity to contribute. Using video conferencing tools...scientists working in different time zones, it’s important that everyone can access data and drafts of manuscripts...sure there is a point person responsible for incorporating everyone’s suggestions into a final version ... spent time in each other’s labs. This is an opportunity for the researchers involved to learn techniques...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...behaving mice to gather the following types of data: Report time-course and concentration of released DA in...optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling during learning and ...moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian...

  4. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... end of the online course, we were given the opportunity to sign up for a writing intensive weekend in...met as a group for sessions covering individual portions of the manuscript. Stephen Blanke, Beronda Montgomery.... Practice writing everyday Overall, the most important instruction the classes taught me was to write...type up the materials and methods. Over time that portion of the paper will be generated. After you complete...

  6. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...

  7. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...transcription.However, this system lacks spatio-temporal regulation, and is difficult to reverse, making...the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none of the...contains BLADE, the PBAD promoter, and a mCherry reporter downstream of the PBAD promoter. To use this plasmid..., including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids from the...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux...makes Keima useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  9. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase to minimize .... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...temperature based on the melting temperature (Tm) of the portion of the primer that hybridizes to the sequence to...used. No matter which polymerase you use, it is important that you sequence the final product. Additional...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

  11. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...finding mentors and, perhaps, asked someone to support your career development as your mentor. How do ...mentoring relationship really productive, the most important step you can take is to set goals to work on together...to help a Mentee reach goals. One of the most important contributions made by a Mentor is to help the ...your creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel...

  12. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...during the pandemic and in the future, depends on support from the scientific community. Deposits of new ... be deposited into repositories, and financial support to maintain repositories’ capacity for storing,...all ways in which the scientific community can support repositories. Repositories are essential for the...

  13. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI...attention for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, ...., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887...

  14. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...back in with the Addgene blog for future award opportunities. Xin Zhou: Fluorescent Light-inducible Proteins...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima ...

  15. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...will include, but is not limited to temporary housing, transportation, living expenses, and necessary reagents...visits an institution to perform research there temporarily. The length of stay could be from a month to ...forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it sounds right...

  17. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ..., the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/...While the Nuffield Council has recently published a report on the social and ethical issues of genome editing...question of “how do we move forward” is the June 2012 report on the ethics of “Novel techniques for the prevention...diseases. The key success of the Nuffield Council’s report is that when it became clear that this assistive...

  18. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of gene editing... and the targeting guide RNA into cells by electroporation. Results from our lab and others (Lin et al...Kim et al 2014) have shown that this technique supports extremely high frequencies of genome cutting and...nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...

  19. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...permanently integrate into the host genome or temporarily express whatever it encodes. This ability to ...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus type 1 preintegration...
Showing: 281 - 300 of 792 results