...activated B cell that produces the antibody of interest with a myeloma cell. This RNA was then used to...Constructs were then verified by both diagnostic restriction digest and colony PCR.   Fig. 1: (A) ... found that the recognition sequence for the restriction enzyme BciVI was present in the aberrant light...

...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...can target the virus to specific cell types of interest. This is a particularly nice feature as organ-...have an idea which ones could be helpful to you. Interested in learning more about a specific virus? We have...

...coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes are highly preferable (and...delighted to engage with potential collaborators and interested audiences. Please visit our team wiki or contact...at the iGEM Competition 2019. He has an active interest in scientific writing and science communication...

...doing after the injunction? We are particularly interested in whether institutions planning to raise salaries...our FOIA requests, we are starting to see very interesting data on how much postdocs are actually getting...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...

...Health Science Center at Houston. Prakash became interested in studying TS when he was studying thoracic ... Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly independent faculty ...channels in the brain.  “I have a long standing interest in the biology of GPCRs and G proteins, with an...

...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...fluctuations due to copy number by introducing genes of interest into the chromosome. But this has its own set ...thereby intensifying repression of the gene of interest and keeping its expression level constant. In ...

...fail. Our laboratory manager would definitely be interested to see which part of her procedures are the most...getting up and running and more time working on interesting biotechnology code.There are valid technical ...Shamloo is a Software Engineer at Addgene and is interested in writing software tools to help scientists....

...them a quick scan for interesting key words and download what seems interesting or relevant. Harness the...keeping up with the literature in your field of interest. Set aside some time each day or week to at least...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...difficult to detect proteins with short half-lives. Interested in using Split-BioID with your research? Plasmids...

...in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al., 2015). In this system...spatially and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The...halves of cGAL can drive expression of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene...

...which may be of particular interest to MoClo users!). If you’re interested in complex Golden Gate Assembly...overhang set by uploading the set with the Type IIS restriction enzyme and thermocycler protocol. It will also...

...potentially signaling a rough road ahead for students interested in leading the process despite the recent advance...students will pursue this newly available avenue. Interestingly, both of these changes share a common thread...understand human disease.  Outside the lab, he is interested in communicating science and in the intersection...

...integrity of your virus, perform a diagnostic restriction enzyme digest and compare it to some reliable... use the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior...24003179. PubMed Central PMCID: PMC3760253. 2. Rohwer, Forest. "Global phage diversity." Cell 113.2 (2003): 141...

...and type of target sites that flox your genes of interest, a powerful FLEx switch can be created for your... considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position...can be employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used...

...chance as entrepreneurs. To make things more interesting, we are working in a new technical discipline...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...doing makes sense. I can support that.” That was interesting. If people could be against GMOs as food but ...

...transferred from B. subtilis to the bacterium of interest following IPTG induction of conjugation genes ... transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...

...with your transfer plasmid, carrying your gene of interest, and the two helper plasmids, commonly called ...Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process, which is what ...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

...tools allowed us to detect and capture proteins of interest to better study them in vivo and in vitro, especially... was to recombinantly append their proteins of interest with unrelated peptides that already had high ...only be added to the C-terminus of proteins of interest and proteins tagged with it needed to be chemically...

...pathway that are of interest to their laboratory. A single mouse click on a gene of interest will direct the...Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing.     References...

...target near the last coding exon of your gene of interest. For ideal use, this gRNA should be cloned into...endogenous promoter and the 3’ UTR of the locus of interest, since the GFP-Puro will be inserted just upstream...nucleases and their applications. It will be interesting to see what new editing possibilities MMEJ can...