...diverse array of processes. The food processing industry, for instance, employs psychrophilic enzymes for...extremely useful benefits for any food processing industry as it can potentially help minimize the incidental...iGEM teams to interact with personnel from the industry and brainstorm with each other. The event also...

...visualization of specific proteins in situ immunocytochemistry is perhaps the most common approach. With...students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this symposium... labeled proteins can be detected using click-chemistry that covalently links the azido containing AHA...

...needs to be cloned into an entry vector. The 2in1 cloning system contains two entry vectors (pUC57-L1L4 and...NC, CN, and CC). The 4 kits also contain the two entry vectors pUC-L1L4 and pUC-L3L2. Find the 2in1 plasmid...

... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...

...slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’... (i.e. RNA replication with no proteins). The chemistry is complex and this work requires an interdisciplinary...forms as we shared millions of years of common ancestry with worms before evolutionary divergence. Nikolaus...

...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...

...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...

...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...

...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...

... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

... populations of synapses and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4...immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry (MS) in HCT116 cells (colorectal cancer). The...isolated and identified with tools like mass spectrometry, providing a snapshot of interacting proteins...

... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...

...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...

...control exceptions were made by the US Bureau of Industry and Security, pursuant to the international controls...-date information on shipping plasmids to your country. Curious about how rabies plasmids are used in ...

...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...