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We narrowed to 771 results for: INA

Showing: 151 - 160 of 771 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf tag; see Figure 2), the authors...al., 2024). Their optimized strategy uses new combinations of PE protein components (Figure 1), an altered...negative-going GEVI that can be used in different illumination regimes with fast activation kinetics and high...://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies for neurodegeneration research   By...-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human...Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results...

  2. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...guests, Melina Fan, Kenneth Fan, and Benjie Chen! Melina Fan is a co-founder of Addgene. Melina received... podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it...University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, ... led business efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to ...

  3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...three codons in eGFP to eliminate fluorescence. This created three inactivated eGFP reporters- eGFP L202...reporter that monitors APOBEC (a family of cytidine deaminase proteins) and Cas9-mediated editing in real time...downstream constitutively active eGFP. To create the inactive mCherry, the lab introduced a 43 base-pair insertion...conversion to GFP after being targeted by a cytidine deaminase base DNA editor. Specifically the BFP mutant (... Figure 3: Targeting pEF-BFP with a cytidine deaminase base editor results in shift in emission spectra...resistance markers, and other enzymes such as Cre-recombinase drastically expanding the base-editing reporter...

  4. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection... diagnose the presence of SARS-CoV-2 RNA, but inadequate access to reagents and equipment has become a... from a sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase...detection by Cas13. This amplification step in combination with the ssRNA reporter enables SHERLOCK to detect..., Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV,...2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science 360...Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F,...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...primary antibody and serum immunoglobulins indiscriminately, causing competition for binding sites. In...antibody will not co-elute with the target protein, eliminating the risk of the primary antibody interfering ...antibodies conjugated within the pores are often inaccessible to their targets. Magnetic beads are solid spheres...need to be verified using alternative methods.  Finally, IP can also be used to study proteins that interact...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...host's replication machinery for elongation and termination. These types of plasmids tend to be high copy...synthesizes a new DNA strand at each replication fork. Termination sequences present in the chromosomal DNA ensure... although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et al., 2018...replication and balance population growth against sustainability. Plasmid DNA replication and regulation Plasmids...which replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since.... Thanks, stringent regulation! This post was originally written by Jason Niehaus in December 2015 and...Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome...

  7. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...nature can inform us on how to create better recombinant vectors. In this blog post, we’ll define plasmid...harness those features to create broad host range recombinant vectors.  A plasmid’s host range is determined...phylogenetic groups (Jain and Srivastava 2013).  Recombinant vectors that are made from broad host range plasmids...host-independent   Building broad host range recombinant vectors Replicons, which consist of a plasmid...broad host range plasmids can be used to create recombinant vectors that can replicate in different bacteria...to incorporating broad host range replicons, recombinant vectors can be modified further to increase the...cloning sites (Keen 1988).  Find broad host range recombinant vectors at Addgene!   References and resources...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal antibody,...different antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit...with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted...structure-guided drug design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ...104 times higher than traditional homologous recombination methods). By using mCherry for selection of ...

  9. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...not. The lack of an exemption for postdocs in the final ruling was, for a lot of us, frankly quite surprising...which leads to many complications, including the inability of some institutions to know exactly how many ...of 10.4%. The Icahn School of Medicine at Mount Sinai in New York is raising the minimum from $43,692 ... funding climate combined with the lack of a sustainable research enterprise, there is no easy way for...cheap trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and...we must make real changes to make the system sustainable. For years, there have been recommendations that... they are necessary to produce a healthy and sustainable research ecosystem and that we cannot ignore ...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...markers and homologous recombination As an alternative, endogenous homologous recombination enzymes can also...enzymes and those that use endogenous homologous recombination. Modifying bacterial genomes using exogenous...exchange requires two sequential homologous recombination events, but because these events are rare, each... ability to grow on sucrose, perhaps by sacB inactivation.  Bacterial toxins for counter-selection In ...mix. Introduction of the strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to ...genetic engineering in bacteria using homologous recombination." Current protocols in molecular biology 106.1...
Showing: 151 - 160 of 771 results