We narrowed to 783 results for: INA

... for the production of offspring that have a combination of the mutations engineered into each of their...journey towards producing mice with your desired combination of mutations. Assuming your parental mice were...crosses. In Part 1, we explained how genetic recombination in mice expressing both Cre and LoxP occurs.... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to... to be homozygous in order for Cre-mediated recombination to affect every copy of your target gene in ... you see in your experiment are a result of recombination at the floxed site, your control should be littermates...29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis...

...vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive or inducible...vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can ...can be used to induce site-specific recombination events and is widely used in the field of mouse transgenics...(Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 under the control...deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time... higher titer of lentiviral particles than the original lentiCRISPR v1. Special note from the Zhang lab...

...neural connectivity (Nassi et al. 2015). The recombinant viruses typically used in neuroscience research...transmitted transsynaptically). This is because the recombinant forms of these viruses typically used in research...G receptor is restricted to presynaptic nerve terminals (Lafon 2005). Therefore, once RABV is released... it is only taken up by the presynaptic nerve terminals of adjacent neurons (i.e., it is taken up by projection... neural tracing. Using these technologies in combination with molecular tools (e.g., optogenetic or chemogenetic...characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. ...Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583...

...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning, in which ...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...modularity — many parts can be assembled into many combinations. Even with current technologies, it can be extremely... name implies, gene stacking is the use of a combination of multiple genes to achieve a desired effect...resistance. MoClo is a great tool to deliver many combinations of different candidate genes at once to identify...

...including "CLAP", combining an N-terminal CLIPf tag and a C-terminal SNAPf tag; see Figure 2), the authors...al., 2024). Their optimized strategy uses new combinations of PE protein components (Figure 1), an altered...negative-going GEVI that can be used in different illumination regimes with fast activation kinetics and high...://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies for neurodegeneration research   By...-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human...Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results...

...Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal antibody,...different antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit...with the multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted...structure-guided drug design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ...104 times higher than traditional homologous recombination methods). By using mCherry for selection of ...

..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...primary antibody and serum immunoglobulins indiscriminately, causing competition for binding sites. In...antibody will not co-elute with the target protein, eliminating the risk of the primary antibody interfering ...antibodies conjugated within the pores are often inaccessible to their targets. Magnetic beads are solid spheres...need to be verified using alternative methods.  Finally, IP can also be used to study proteins that interact...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...

...three codons in eGFP to eliminate fluorescence. This created three inactivated eGFP reporters- eGFP L202...reporter that monitors APOBEC (a family of cytidine deaminase proteins) and Cas9-mediated editing in real time...downstream constitutively active eGFP. To create the inactive mCherry, the lab introduced a 43 base-pair insertion...conversion to GFP after being targeted by a cytidine deaminase base DNA editor. Specifically the BFP mutant (... Figure 3: Targeting pEF-BFP with a cytidine deaminase base editor results in shift in emission spectra...resistance markers, and other enzymes such as Cre-recombinase drastically expanding the base-editing reporter...

...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection... diagnose the presence of SARS-CoV-2 RNA, but inadequate access to reagents and equipment has become a... from a sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase...detection by Cas13. This amplification step in combination with the ssRNA reporter enables SHERLOCK to detect..., Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV,...2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science 360...Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F,...

...host's replication machinery for elongation and termination. These types of plasmids tend to be high copy...synthesizes a new DNA strand at each replication fork. Termination sequences present in the chromosomal DNA ensure... although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et al., 2018...replication and balance population growth against sustainability. Plasmid DNA replication and regulation Plasmids...which replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since.... Thanks, stringent regulation! This post was originally written by Jason Niehaus in December 2015 and...Leonard, A. C. (2018). Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome...