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We narrowed to 792 results for: LAT

Showing: 451 - 480 of 792 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...fractionation kit or a lysing protocol developed for isolating proteins from a specific compartment. If your ...blockers, you can run a comparison of different formulations instead of concentrations. Most labs use protein-based... For nitrocellose membranes, you can even give gelatin a shot.  Remember that protein-based blockers degrade...in an assay, meaning that your normalization calculations will not be correct, and you’ll need to adjust... Splice variants Check literature Posttranslational modifications Check literature Highly...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...certain region or calculate a colocalization factor to assess the spatial relationship between the targets...need more signal, another strategy is to use a biotinylated secondary antibody then label that with fluorescently-conjugated... other antibodies are disrupting the binding of later targets, the sample is being damaged by all the ...

  3. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...harassment Isolation – including preventing access to opportunities, physical or social isolation, withholding... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...regretted it when the same thing happened to her a year later. I hear too many science trainees get warnings and...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...AAV selection, there are three important factors related to location. Location (1): What tissue? Location...tools for neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene...and Mark A. Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene...

  5. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...sequence but not the amino acid sequence of the translated protein.  These changes can be 5’ or 3’ of the...strains, expression of exo, beta and gam is tightly regulated by the endogenous phage promoter pL and repressor...for a mobile recombineering system, but tight regulation of expression is required for a successful experiment...endogenous red promoter pL and the cI857 repressor to regulate expression. An antibiotic can be used to select...

  6. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... day or a week planning an experiment, even a relatively simple experiment. However, by taking this time...surprising findings when studying a seemingly unrelated problem. However, you have finite time and resources...background slides. If you don't have much new data, speculate on what you hope to accomplish and how your research...satisfying. Finally, many PFs are in committed relationships and/or have started a family. Those people are...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...hope that these perspectives, the optimism they encapsulate, and the solutions they promote help motivate...infertility or to argue against unnecessary clinic regulations, evidence-based research is vital in combating...Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan, where programs like...worried that, because we were speaking out against legislators and because the march had been so well-publicized...

  8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...The machine is useful for distinguishing cell populations that have been tagged with different fluorescent...The optimization of GFP and its derivatives by the late Roger Tsien changed everything. For the first time...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering..."Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior." Science...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All that is required... UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid finds the...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...applications." Current Chemical Genomics and Translational Medicine 3.1 (2009). PubMed PMID: 20161833. ...

  10. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...LinkedIn. We are also exploring other social media platforms with active scientific communities and welcome...functionality and features that previously made the platform a mainstay of conversation in the scientific community...considering a gradual move off X as a response to major platform changes, such as limiting access and increased...

  11. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources! Ready...gave to me: Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 ...variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st day of CRISPR, Addgene gave...

  12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...cells. The goal is to use cells that can be easily isolated from donors. Apart from fibroblasts, human keratinocytes...epithelial cells from urine are some of the easily isolated somatic cells that have been reprogrammed to iPSCs...the reprogramming factors) More Chances of accumulation of harmful mutations More, due to selection ...from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci...

  13. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Abhi Aggarwal gets the reader up-to-date on the latest version of the iGluSnFR glutamate sensorRUBY-Red...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

  14. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...the scientific community. Here are a few virus-related protocol videos that may help to save you time ...AAV preparation. For detailed instructions and calculations on how to prepare a plasmid stock used to generate...with tissue cultures. To keep up on all of the latest protocol videos, lab tips, and more video content...

  15. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...Früh (@Michelderapfel) December 19, 2022   Congratulations to all our #DeckTheLab contestants!           ...tubes, hang up your pipettes, and seal up those plates – it’s time to turn your attention to decorating...decorations and that you’re following all safety regulations. That’s one sticky situation our customer service...

  16. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...t cross the blood-brain barrier. By lowering circulating levels of kynurenine in the body, the brain is...Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience ...doi: 0.1016/j.cmet.2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... CRISPR, there's a pretty good reason for that. Lately, significant advances in the understanding and ...another very different bit of CRISPR news was circulating. Scientists at North Carolina State, in the labs...as an even more precise and useful tool. A week later, researchers at the Broad Institute and MIT published...

  18. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...incubation step and go straight to plating if your transformed cells are being plated on agar with ampicillin selection...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make sure ...

  19. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...that really needs it, but then it might to be too late,” Sacher says. As the labs in the directory step...phages or with phages targeting organisms closely related to the clinical sample. For treatment, it is best...phages for clinical use. There are also different regulations from country to country when it comes to administering...

  20. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...spooky science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their...to female. Then, it detaches from the gills and latches onto the base of the tongue where it pierces the...References Biron, D. G., et al. "Behavioural manipulation in a grasshopper harbouring hairworm: a proteomics...

  21. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    .... With this information in hand, you can then formulate a plan to further develop your transferable skills... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...10.1016/j.molcel.2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong...

  22. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...on the Central Dogma of Biology, I would stop by later this year when it is displayed!).  The Addgene themed... intention of ending the celebration. The party later moved to State Park, where I had a great (maybe ...helped bring it to fruition. The concept, though relatively simple, changed the course of scientific research...

  23. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from.... In total, three MCS regions permit multiple regulatory elements to be inserted, further customizing ...

  24. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only...Multiple new site-specific recombinases for use in manipulating animal genomes. Nern A, Pfeiffer BD, Svoboda...Drosophila cells by estrogen-dependent activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 ...

  25. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...'s more than 50 antibodies target neuroscience-related proteins, but we recently added several broadly...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

  26. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...where the eosinophils release cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (... once bound to an antibody, can act as immune modulators, shaping the immune response by driving particular...Presta LG, Ravetch JV. Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat...

  27. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments, just about...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to... husband Enzo will spend a few days editing and later looking for songs and sounds to synchronize with...

  29. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...aminoglycoside Binds 30S ribosomal subunit; causes mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

  30. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...access to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant...vector distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids ...
Showing: 451 - 480 of 792 results