...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...effective reporter for adenine base editors, that make A-to-G edits in the genome (Gaudelli et al., 2017...

...edits but the double strand breaks it introduces makes it difficult to perform two or more integrations...targeting another site of interest can be used to make other edits. Once you have all the edits you want...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...the list of antibiotic options doesn’t end there. Make sure you read the instructions for whatever antibiotic...kingdoms of life. A bit of careful design will help make your experiment with shuttle vectors a success! ...

...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

...microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within living...approaches and appropriate controls are required to make this method useful for dynamic experiments in which...

...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the...on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working...

...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...alleles had unwanted insertions/deletions, therefore making those alleles useless for our studies. If one now...

...contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third...

... available as lentiviral plasmids if you want to make a stable cell line. This design allows highly localized...bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t overdo it! The moderately-enhanced...

...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...protein research institute, partnered with Addgene to make our collection of integrin antibodies available ...

...recombinase. The XFPs in Flybow are membrane-tethered, making this system useful for visualization of cellular... optimize it for tissue clearing, a method that makes tissues, such as the brain, transparent so that ...

...easy, but the risk of PCR-introduced mutations can make alternative approaches more favorable if you want...restriction sites. Alternatively, if you want to make your protein non-functional, you may simply introduce...

... unmethylated DNA, and more. Many mutations that make these features possible are present in most commercial...commercial strains, especially mutations that make major improvements such as those that increase plasmid...

...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...

... disease states, and targeting the epigenome may make certain processes, like cellular reprogramming of... likely cell type- and context-dependent, it may make sense to try multiple CRISPR tools when setting ...

...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay attention...knocked down or knocked out. If you are interested in making your own knockout line for validation, please view...

...exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees... modification of interest, it would be unwise to make conclusions on the basis of the activity of a single...

...recombination-mediated genetic engineering.  It can be used to make an assortment of modifications: insertion and deletion...with 4-5 silent changes in the wobble codons, i.e. make changes to the third base pair of the adjacent 4...

...the parental animal, are rescued progeny, which makes screening extremely rapid. This stringent selection...knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more efficient. Pure...

...switch from a dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...

...during chemical synthesis of these molecules. To make large amounts of pure product in an affordable way...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested in recombinant...

...immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region and part...epitopes on the protein. However, each animal will make antibodies against different protein epitopes and...

...controls needed to pressure-test your FRET assay to make sure it is working? This article addresses these...FRET experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa ...

...ability to leverage the prestige of the employer makes EB-1Bs a very attractive option.    Your Expert ...a career search. Doing these simple things will make it easier for you to find and organize all of the...

... plateau stage after exponential amplification, making it difficult to properly analyze your data.  To...understanding how the analyses work - you do need to make sure the assumptions in your dataset match the assumptions...

...The ease and flexibility of the LACE technology makes this system widely accessible for many potential...interest within the INT location. One plasmid to make note of is the INT construct bearing the "Bunch ...previous studies, and provide new insights into the make-up of multiprotein complexes like the EPC1 complex...

... no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  in E. coli and...the solution to this inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective ...