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  12. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more accurately...

  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2)...

  16. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which...

  21. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...

  22. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...how will you use these systems in your research? Cary Valley contributed to updating this article. References...

  23. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... manual droplet generator, you will have to be careful about transferring your droplets from the droplet...

  24. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ...

  25. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated...

  27. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates...
Showing: 451 - 480 of 523 results