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Showing: 451 - 480 of 535 results
  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting, unlike other well-characterized...
  2. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources Additional...
  3. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat...
  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more accurately...
  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2)...
  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...
  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...
  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...
  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...how will you use these systems in your research? Cary Valley contributed to updating this article. References...
Showing: 451 - 480 of 535 results