... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate... and plasmids. To encourage collaboration and education in protein science, IPI hosts events and training...subtype-specific β1 integrin mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467...

...setting. CRISPR is often used for animal germline modification, to repair or add in disease-causing mutations...that AAV-CRISPR does not cause obvious germline modification, an important finding given the controversy ...Blog Learn How CRISPR Could Be Used to Treat Cataracts and Cystic Fibrosis Read How Homology Directed...

...tests  Statistical tests fall into two general categories: parametric and non-parametric. In this blog ... exist somewhere within your data; it does not indicate which groups the differences exist between. For...that matter. However, this is oftentimes an oversimplification. If your independent variables are affecting...

...immune response to the development of tumors is a complicated and dynamic phenomenon that is interestingly ...effects, damage healthy cells, and often cannot eradicate metastasis or eliminate the probability of a relapse...based on their level of progression, anatomical location, characteristic (solid localized tumors or systemic...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

...antibody collection, as well as access to IPI educational resources developed to support use of their antibody... Addgene also offers a growing collection of educational resources on our website and blog, including ...

...these tools over the years, the amount of CRISPR educational resources hosted on our website and blog have...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....et. al. (2022) A plasmid-based system for locating and containing escaped flies. Nature 4:1-22. https:/...

...With barcode tracking, you will always be able to locate materials quickly and easily. Learn more about ...Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos Page 5 Science Rap...

...easy tech transfer process to our website and educational resources. We've got twenty years of experience... you know you can search for plasmids from a publication by pasting the paper's title into the Addgene...

.... I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the foundations...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

...Twitter and has helped us update many of our educational resources. Listen to hear all about Professor...speeds science. Chapter 8 - The importance of communicating research findings through social media and other...

...Born to a family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best... 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to go into...

... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...

...Intro Chapter 1: Who is Crystal Shih? Crystal's educational background and position at Novartis. Chapter ...Chapter 7: How Crystal developed her science communication skills and how they've helped her at Novartis...

...plasmids! Each chose their favorite plasmid and replicated it. Can you guess each plasmid? Our lab paid...creative hijinks last year. They showed us their dedication to great customer support by dressing up as our...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...

...fantastic posts in October. Click on the links below to catch up on ways to use CRISPR in plants, to learn techniques...Writing 5 Reasons to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc ...

...news, and awards that we offer there. Each topic category contains a overview page to help you get aquatinted...the blog articles come up as well as their topic category. New author pages to find articles from your favorite...

...decorations yet again. This year, we had two different categories: one for decorations in the lab, and one for ...Addgenies voted, and selected three winners in each category. Congratulations to the winners! Deck the Lab:...

...skipping the preparation steps of production, purification, and titering. Our newest viral vector service...vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670 AAV1 Control...

...editing technology, Cpf1. Written by our Science Communications Intern (and soon to be Addgene Scientist!) ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...

... eukaryotic cells). pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones...considerations  If your insert contains a mutation or modification, this should be included in the plasmid name...

...office – previously at 1 Kendall Square, but newly relocated to 75 Sidney Street. Then the plasmids travel ... thank you. 500,000x Thank You! Addgene's new location: 75 Sidney St, Suite 550A, Cambridge, MA 02139...

...window onto communication between the muscle and brain, which may have therapeutic implications, say Hyo ...

...of interest. 5. Use a silica spin column for purification of the samples you send for DNA sequencing If...requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all inclusive...

...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...caused by genetic abnormalities, targeted genetic modification in monkeys is invaluable for the generation ...

...at target sites. Read the paper in Nature Communications Find the Cas12b plasmids  Plant base editing...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...

...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two... small claims court summary process. Julie is dedicated to volunteerism and ran the Mayor’s Midnight Sun...

...they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ... a Lab and a Project That’s it for now. As I indicated in the opening paragraph, I’m currently on my ...

...accessible and reliable. We continue to expand our educational content supporting the tools in our collection... growing community on Bluesky, our expanding educational resources, and our commitment to global accessibility...

...researcher may only know how to prepare phage for one application but not another or they may have only used phage...Directory expands, it can also fill this role by cataloging and archiving information on individual phages...

... into a pulsating display imitating a moving caterpillar. The worm controls the snail’s behavior, forcing...exposed to predators. Hungry birds spots the “caterpillar” and then poke out the snail’s eyes and ingests...

...determining meaningful solutions. PI-trainee communication builds a positive lab culture Although they ... communication...

...problem is that scientists sometimes forget how to communicate about their work with non-academic people.” Daniel... new method to explore the inner structures of delicate plants and other organisms without destroying ...

... helps you reach your goals Listens well and communicates clearly and openly Gives honest feedback without...lab. If only every science trainee had such a dedicated person guiding the start of their career. “I want...

...expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus...methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you have a new...

...important. It needs to express value, clearly communicate exactly what a site offers to their users, and...hug items closer and do so more animatedly. To communicate that sense of community, Blugene will regularly...