We narrowed to 814 results for: Ran;

...paid on fellowships instead of grants such that PIs no longer save grant money by paying relatively low...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan...

...using the actual tool that you ordered and not a random aliquot pulled from a freezer. As you’ll see in...review our experimental designs and we need to be transparent about the reasons we alter these designs if we... wherein researchers can submit designs and transparently reveal them upon completing an experiment. There...

... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...

...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...

...Subscribe to the blog to stay in the know!     No pranks, just fun and games this April Fool’s Day on the... to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each photo, the pet...

...competitive field. Chapter 2: Wei Leong Chew's swift transition from graduate work to an independent position... Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: Wei Leong Chew's lab...

...Troubleshooting Viral Transductions Getting the Most from Your Lentiviral Transduction Additional Resources...

...the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History Rap Battle...telling following the unfortunate story of Rosalind Franklin and her incredibly important role in solving the...

...100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown announced...substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will allow us to...

...field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook....other Cas proteins continue to expand the targeting range. CRISPR can be difficult to deliver. Not all cells...

...Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4 - Developing...ensuring that success Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global...

...diverse needs of our community and support a wide range of experimental designs. Here are nine great additions...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

...Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf... care package from Addgene. We hope all of our entrants had fun decorating with their labmates! ...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...

...scientific breakthroughs, reagents, and know-how to transcend national borders and help people around the world...accelerate biology research in the labs they’re granted to, as well as shine a light on some of the great...

...light chains and two heavy chains. The ends of the branches contain the antigen binding sites and are highly...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...

... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...

... insufficient (or no) emphasis on developing transferable skills to enable pursuit of these diverse opportunities...with independent career organizations to get the grants and programs they need such as at the Postdoc Career...

...glowing protein could be used to measure gene transcription, as well as to track protein localization. And...bioluminescent, some types of corals and anemones are vibrantly colored. He hypothesized that GFP-like proteins...

...that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12 do for RNA and dsDNA, respectively...can cleave fluorescent probes of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this...

...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged ...A., Crosby, K., Harvey, K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., … Laflamme, C. (2024...

...Editor's Choice Blog Posts As I’m sitting in the San Francisco International Airport listening to the Lion King...back from a very successful Outreach Trip in San Francisco and would like to give my thanks to all the Addgene...

...Office of Science and Technology Policy issued a memorandum calling on Federal agencies with over $100 million...(66 percent) gave preference to journal access granted via a public repository or authors’ own web sites...

...then formulate a plan to further develop your transferable skills and reach your career goals.      Although... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ...

... her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used ...

...and see if we should start celebrating papers and grants too with pizza or pub.” The Cepko Lab celebrates... all work and no play Although hard work and perseverance are required to achieve success in the lab, ...

...community response initiatives. Accelerate the translational impact of research Universities have also been...creating research synergies and accelerating the translational impact of research activities. By streamlining...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

...with a detailed training schedule that helped me transition easily. Training and responsibilities Through... the “9-5” was not as scary as it seemed. The transition into a traditional work environment was aided...

...improve search and navigation, while also updating brand messaging, as well as simplifying the page, and ...wanted to make sure our friend matched our core branding. Blugene needs to be friendly, personable, fun...

...many applications, the most well-known being transcriptional activation/repression. dCas9 has also previously...for example, the visualization of chromosomal translocations or chromosomal shattering (chromothripsis)....

...from in the green, red, yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all...F-Actin to mitochondria, from the nucleus to cell membrane, this collection has fluorescently tagged versions...

...sonification. Though it helps capture weaker or more transient DNA-protein interactions, the reliance on fixation...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...

...beyond your immediate field, fostering a diverse range of perspectives and sparking innovative thinking...Making the Leap to Industry." He successfully transitioned to industry after his PhD and a couple of postdocs...

...from the DNA. These apurinic sites if in double stranded DNA are repaired by base excision repair, but ...but can lead to mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest...

...cultivates trust between coworkers and that trust then translated into this incredible experience.  Of course, ...to-dos, I also found time to go out to sushi restaurants for lunch with coworkers and visit the office...

...includes an antibiotic resistance marker. After transformation, plate the cells on medium containing the corresponding...selection will select for cells that have been transformed with the plasmid backbone (with or without your...

...voltage indicator: JEDI-2P  by: Brian O'Neill  The François St-Pierre lab and collaborators have successfully...photoactivation of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1...

...of University of Michigan says, “Addgene has transformed the way in which we share plasmids and other ...shipping logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors...