We narrowed to 872 results for: fer

...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (... using in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...Gel purify your PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

...field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors. Perhaps most ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

...sequences and lineages, FRET efficiencies, additional references, etc...) and a more robust database structure...address).  One may also simply attach important references that serve to further characterize a protein ...Currently, all submissions also require a DOI referencing primary literature, and every effort is made ...

...both individual and community science projects, we offer classes, workshops, book clubs, seminars, and social...the data to characterize the microbiomes of the different samples. The goal is to not only learn something...something about the microbial diversity in different soil conditions/locations but to engage the local community...

...assembly? In the context of cloning, DNA assembly refers to a method of physically joining multiple fragments...generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, there is no...assemblies and improvements in these methodologies offer streamlined and simplified alternatives to traditional...

...used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...

...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input...constructs like plasmids. The plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger...

...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...ask questions if you don’t understand something. Offer to help out wherever you can and don’t forget to...

...media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative word...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...

...     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and the New Research...sometimes we benefit and sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator...follows an intriguing cast of characters from five different families over several millennia as their paths...

...developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase reporters. In...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...

...there are two overlapping, valid critiques being offered of He’s work: CRISPR/Cas9 genome editing is not...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...self-police” or whether the media and academic conference response to He created a positive feedback loop...

...sure to thank everyone for their participation and offer encouragement for the next steps (see “action items...employee’s workload, updates on current projects, and offer feedback. The employee should ask for input on projects...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

...blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including how...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working.... This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin...

...components are still there in real life.  Certainly referring to any DNA within the context of a nucleus as ...chromosomal domains, or how stretches of DNA on different chromosomes interact with each other. Chromatin... Note that histone genes frequently have many different variant names, not all of which can be listed ...

...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

...’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused ...opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized programs...point into further STEM exploration.  Further offerings for both STEM teachers and STEM-loving students...

...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

...that pathways projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...

...nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles, the functional...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...

... of interest). Mesophilic microbes are highly preferable (and successful) as host systems since their ...to guess. Think about it like winter tires in different conditions: the more flexible rubber of winter...reagent repositories and the myriad facilities offered by them which help foster a more productive and...

... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...constantly reviewing the resources we currently offer to identify gaps and add to them as techniques evolve...

...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as your experimental RNA. ...

...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM... part of my job is getting to talk to so many different people! I communicate with customers, software... get software product management internships, preferably on teams that run within the agile framework ...

...synthesis, and multifragment assembly methods are preferred. No such restriction applies to deletions; using...common methods for site directed mutagenesis   References 1. Trehan, Ashutosh, et al. (2016). “REPLACR-... on Addgene.org Browse Our Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find...

...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...the Iron Response Element (IRE) RNA motif of the ferritin light chain RNA transcript. IRE motifs are bound...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

...difficult for an individual lab to produce - Hybridomas offer an alternative and renewable source of monoclonal... their use. Each RANbody is detectable by two different tags: a reporter and an HA tag. The reporter comes... link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-...

...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper localization...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular... Jennifer Tsang...

...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous... a scientist looking for that perfect fit!  References and Resources Additional resources on the Addgene...AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and ...

...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...

...replicates would have different labels - i.e. they would stochastically arise from different cells throughout...development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal...

...because it “causes recombination” (although later referred to as the “cyclization recombinase”), is a 38 ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder... cure research and science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher...

...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...portal. The plasmids should be functional in many different human cell types, and while most of the genes ...Allen Institute website at alleninstitute.org. References 1. Roberts, Brock, et al. "Systematic gene tagging...

...updated in 2023 by Susanna Stroik.  References and Resources References Yuxuan Wu et al. "Correction of a...frequency isn’t high enough to make a physiological difference, then the treatment will not work in a meaningful...