960 results

Learn how to use recombinase-based state machines from the Lu lab to monitor cellular events and regulate gene expression.

July 28, 2016, 2:30 p.m.

Learn about the importance of considering sex and gender in your research.

July 21, 2016, 2:30 p.m.

Learn how to target donor and acceptor fluorophores for FRET experiments.

July 19, 2016, 2:30 p.m.

Learn how Cpf1 stacks up against Cas9 and NgAgo

July 14, 2016, 2:30 p.m.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

July 12, 2016, 2:30 p.m.

Addgene recently attended the ASM Microbe 2016 conference in Boston. Check out what we learned!

July 7, 2016, 2:30 p.m.

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

June 30, 2016, 2:30 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

June 23, 2016, 2:30 p.m.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

June 21, 2016, 2:30 p.m.

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

June 15, 2016, 1:30 p.m.

We've recently curated a Microbiology Plasmid Collection. Learn what you can find in the collection in this post.

June 13, 2016, 7:08 p.m.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

June 9, 2016, 2:30 p.m.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

June 2, 2016, 2:30 p.m.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

May 31, 2016, 3:30 p.m.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

May 26, 2016, 2:30 p.m.

In this podcast, learn about Kingdom Kwapata, a Malawian researcher currently developing transgenic crops and new means to produce biofuels.

May 24, 2016, 2:30 p.m.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

May 19, 2016, 2:30 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

May 10, 2016, 2:30 p.m.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

May 5, 2016, 2:30 p.m.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

May 3, 2016, 2:30 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.

April 19, 2016, 2:30 p.m.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

April 14, 2016, 2:30 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

Addgene is teaming up with nonprofit Seeding Labs to provide plasmids to researchers in developing countries.

April 5, 2016, 2:30 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

March 24, 2016, 2:30 p.m.

Perturb the activity of 17 different signaling pathways using the cancer pathways ORFs kit.

March 22, 2016, 2:30 p.m.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

March 17, 2016, 2:30 p.m.

Can't find a plasmid at Addgene? Suggest new plasmids for the repository by tweeting at us of filling out our "Suggest A Plasmid" form here.

March 16, 2016, 5:30 p.m.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

March 15, 2016, 2:30 p.m.

Learn how Addgene works with you to increase your work's visibility and citations through efforts to organize and curate the repository.

March 10, 2016, 3:30 p.m.

We're recruiting contributors to the Addgene Blog to expand the educational reach of our Plasmids 101 series. Sign up to contribute!

March 2, 2016, 3 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

Feb. 25, 2016, 3:30 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Feb. 18, 2016, 3:42 p.m.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Feb. 10, 2016, 3:30 p.m.

Tired of meetings? Read this blog post for tips on how to run an enjoyable and productive meeting.

Feb. 9, 2016, 3:30 p.m.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Feb. 4, 2016, 3:30 p.m.

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Feb. 2, 2016, 5 p.m.

Three studies recently showed that CRISPR can be used to treat Duchenne muscular dystrophy in mice. Learn about the work and obstacles to application.

Jan. 26, 2016, 3:30 p.m.

Dr. Michael Koeris is cofounder Biotech startup, Sample 6, and a member of the Addgene Board. Listen to his journey from grad student to entrepreneur.

Jan. 21, 2016, 4:52 p.m.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Jan. 14, 2016, 3:30 p.m.

Addgenie Emma Markham describes her winding career path and provides advice on how you can find a great job after undergrad.

Jan. 12, 2016, 3:30 p.m.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Dec. 30, 2015, 3:30 p.m.