We narrowed to 812 results for: APP

1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

2. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

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   Jan. 8, 2019, 1:54 p.m.

   ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

3. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...s advice to tap into the online conversations happening around a scientific conference, whether you are...

4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ... markers make it easy to spot flies with the appropriate transgene. One such marker is the white gene,...

5. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

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   July 6, 2017, 1:59 p.m.

   ...interactions, cancer immunotherapy, as well as new approaches to reprogramming tumor-associated inflammation...

6. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...can be detected is 5x greater than our previous mapping software and now includes Type IIS restriction ...

7. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

8. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...

11. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...realize that the bench isn’t where they will be happiest. What is software product management, and how ...

12. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type

    Blog Post

    Published

    Sept. 15, 2020, 1:15 p.m.

    ...other ways to choose restriction enzymes for this approach. If you’re planning to go this route, check out...

13. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...discussed in this post, voltage has been adjusted appropriately and compensation performed. Before you read ...

14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...

15. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1 subclass (Manning...

16. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ... to be stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from ...

17. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...relationships that are memorable. It isn’t always appropriate to walk up to someone much more senior than you...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ... cells to the microenvironment. This mechanism happens for both short and long distances. When it comes...

19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ... assays Long-lived protein degradation: This approach measures the degradation rate of radio-labeled...

20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...densely populated areas. This assumption does not appear to hold. There are institutions in Boston - Massachusetts...

21. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...take place in a tissue culture dish but can also happen in real-time inside a mouse’s brain.  But the brain...

22. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...the skin making it important to study what is happening within the keratinocytes. “We hope to use CRISPR...

23. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression...

24. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ... glycerol stocks, so make sure to select the appropriate service and read the submission instructions....

25. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...BEST) awards, which are designed to support new approaches that address the need to train our young scientists...

26. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...there’s a lot of control spatially as to what’s happening at different points within the wood. There are...

27. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...Industry Professionals”. This larger shop might appeal to some although most scientist coaches can help...

28. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...

29. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ... also used to teach students about restriction mapping and western blotting. Students who participate ...

30. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...influence (non-homologous end joining, or NHEJ), in approximately one percent of cases, the stem cell will seek...

31. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...choose essentially any background, but two common approaches are either a solid color, or a “themed” background...

32. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

33. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...MiSeq and set up the run making sure to note the appropriate barcode for each sample. About 24 hours later...

34. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody pairs. Because the...

35. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression can...

36. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...institutions are not local but are national issues. I applied and was successfully granted a travel award to ...

37. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...

38. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics software to...

39. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you...

40. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ... couple of weeks now”. I had no idea this was happening even though I met with X on a weekly basis! The...

41. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

42. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    Published

    Feb. 4, 2016, 3:30 p.m.

    ...those flames about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication...

43. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell...

44. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

45. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits...

46. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    Published

    Sept. 6, 2022, 1:15 p.m.

    ...this information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV...

47. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents...

48. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

49. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ...

50. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...